Mib 1

Manufactured by Thermo Fisher Scientific

Sourced in United States

The MIB-1 is a lab equipment product from Thermo Fisher Scientific. It is designed for the detection and quantification of the proliferation marker MIB-1 protein. The MIB-1 product provides a reliable and standardized method for researchers to analyze cell proliferation in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Rabbit anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Confirm anti er sp1, by Roche (1 mentions) Confirm anti pr 1e2, by Roche (1 mentions) Herceptest, by Agilent Technologies (1 mentions) Ventana benchmark xt, by Roche (1 mentions) Labvision autostainer 480s, by Thermo Fisher Scientific (1 mentions) Histofine simple stain mouse max po, by Medac (1 mentions) Super sensitive link label ihc detection system, by BioGenex (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Diaminobenzidine dab, by Agilent Technologies (1 mentions) Mu392a uc, by Abcam (1 mentions) Alexa 594, by Thermo Fisher Scientific (1 mentions) Click it plus alexa fluor 555 picolyl azide toolkit, by Thermo Fisher Scientific (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Ab5392, by Abcam (1 mentions) Goat anti sox2, by Santa Cruz Biotechnology (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Rabbit anti sox2, by BioLegend (1 mentions) Mrb 435p, by Abcam (1 mentions)

4 protocols using mib 1

Immunohistochemical Analysis of CYC1, ER, PR, and HER2

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit polyclonal antibody for CYC1 (PA5‐25257) and mouse mAb for Ki‐67 (MIB1) were purchased from Thermo Fisher Scientific (Waltham, MA, USA) and Dako (Carpinteria, CA, USA), respectively. The antigen–antibody complex was visualized with DAB solution and counterstained with hematoxylin. We used human tissue of the stomach as a positive control based on a data sheet of CYC1 antibody by Novus Biologicals (http://www.novusbio.com/CYC1-Antibody_NBP1-86872.html#dsTab0) and normal rabbit IgG instead of the primary antibody as a negative control for CYC1 immunostaining in this study.
Immunohistochemistry for ER (CONFIRM anti‐ER [SP1]; Roche Diagnostics Japan, Tokyo, Japan) and PR (CONFIRM anti‐PR [1E2]; Roche Diagnostics Japan) was carried out with Ventana Benchmark XT (Roche Diagnostics Japan), and that for HER2 was carried out using HercepTest (Dako).

Chishiki M., Takagi K., Sato A., Miki Y., Yamamoto Y., Ebata A., Shibahara Y., Watanabe M., Ishida T., Sasano H, & Suzuki T. (2017). Cytochrome c1 in ductal carcinoma in situ of breast associated with proliferation and comedo necrosis. Cancer Science, 108(7), 1510-1519.

+ Open protocol

+ Expand

Xenograft Tumor Immunohistochemistry Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Xenograft tumors of mice treated with either TW-37 or control were paraffin embedded. All tumors were clinically and pathologically identified as being the primary and only neoplastic lesion. Briefly, 3-μm-thick sections of formalin-fixed paraffin-embedded (FFPE) tumors were deparaffinized, and antigen retrieval was performed by boiling the section in citrate buffer at pH 6 or EDTA at pH 9 for 20 min. As primary antibody Ki67 (mib-1, 1:100, pH 6, Thermo Scientific, Waltham, MA, USA) was used. Corresponding secondary antibody detection kits for reduced background on murine tissue were used (Histofine Simple Stain Mouse MAX PO, medac) and stained on an automated stainer (LabVision Autostainer 480S, Thermo Scientific). For cleaved caspase 3 (Cell Signaling Technologies, Danvers, MA, USA) staining of paraffin sections, antigens were retrieved with EDTA buffer (1 mmol/L EDTA, pH 8.0), peroxidases blocked 10 min in 3% hydrogen peroxide, and the antibodies were diluted in Tris-buffered saline containing 1% bovine serum albumin and 5% normal goat serum 1:200. The histochemistry was performed with Super Sensitive Link Label IHC Detection system (BioGenex, San Ramon, CA, USA) and visualized with diaminobenzidine (DAB; Dako, Giostrup, Denmark).

Klenke S., Akdeli N., Stelmach P., Heukamp L., Schulte J.H, & Bachmann H.S. (2019). The small molecule Bcl-2/Mcl-1 inhibitor TW-37 shows single-agent cytotoxicity in neuroblastoma cell lines. BMC Cancer, 19, 243.

+ Open protocol

+ Expand

Immunofluorescence Staining of Flavivirus and Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed with 4% PFA for 20 min at room temperature, blocked in Mg²⁺/Ca²⁺ free PBS containing 5% horse serum and 0.3% Triton-X for 1 h at room temperature, and then incubated with primary antibody at 4 °C overnight. The following primary antibodies have been used with given dilutions: mouse anti-Flavivirus group antigen antibody (ZIKV E) (1:2000; Millipore, clone D1-4G2-4-15), mouse anti-Ki67 (1:200, DAKO, MIB-1), rabbit anti-Ki67 (1:500, Thermofisher, SP-6), rabbit anti-cleaved caspase-3 (1:1000, Cell Signaling Technology, Asp15), mouse anti-CDX2 (1:100, BioGenex, MU392A-UC), and rabbit anti-KRT7 (1:500, Abcam, ab119697). The secondary antibodies include donkey anti-mouse, goat, rabbit or chicken secondary antibodies conjugated with Alexa-488, Alexa-594 or Alexa-647 fluorophore (1:500, Life Technologies). Nuclei were counterstained by DAPI. EdU incorporation was analyzed with Click-iT™ Plus Alexa Fluor™ 555 PicolylAzide Tool kit (Thermofisher, C10642).

Tan L., Lacko L.A., Zhou T., Tomoiaga D., Hurtado R., Zhang T., Sevilla A., Zhong A., Mason C.E., Noggle S., Evans T., Stuhlmann H., Schwartz R.E, & Chen S. (2019). Pre- and peri-implantation Zika virus infection impairs fetal development by targeting trophectoderm cells. Nature Communications, 10, 4155.

+ Open protocol

+ Expand

Immunofluorescence Staining of Cells and Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed with 4% paraformaldehyde for 20 min at room temperature (RT) and blocked in a solution of Mg²⁺ and Ca²⁺ free PBS containing 5% horse serum and 0.3% Triton-X for 1 h at room temperature and followed by incubation with primary antibody at 4°C overnight. The following primary antibodies and dilutions have been used in this study: mouse anti-Flavivirus group antigen antibody (ZIKV E) (1:2000; Millipore, clone D1-4G2-4-15), mouse anti-Ki67 (1:200, DAKO, MIB-1), rabbit anti-Ki67 (1:500, Thermofisher, SP-6), rabbit anti-cleaved caspase-3 (1:1000, Cell Signaling Technology, Asp15), goat anti-SOX2 (1:100, Santa Cruz, sc-17320), rabbit anti-SOX2 (1:200, Biolegend, N-term), mouse anti-NESTIN (1:1000, Neuromics, MO15012), rabbit anti-TUJ1 antibody (1:500, Covance, MRB-435P), and chicken anti-MAP2 (1:1000, Abcam, Ab5392). The secondary antibodies include donkey anti-mouse, goat, rabbit or chicken secondary antibodies conjugated with Alexa-Fluor-488, Alexa-Fluor-594 or Alexa-Fluor-647 fluorophore (1:500, Life Technologies). Nuclei were counterstained by DAPI.
The immunohistochemical analysis of mouse tissues was performed on cryosections. In brief, the tissues were fixed overnight with 4% PFA at 4°C, sunk in 20% sucrose, embedded in OCT, and then sectioned at 30 μm for immunostaining. The primary and secondary antibodies were used as described above.

Zhou T., Tan L., Cederquist G.Y., Fan Y., Hartley B.J., Mukherjee S., Tomishima M., Brennand K.J., Zhang Q., Schwartz R.E., Evans T., Studer L, & Chen S. (2017). High Content Screening in hESC-Neural Progenitors Identifies Drug Candidates that Inhibit Zika Virus Infection in Fetal-like Organoids and Adult Brain. Cell stem cell, 21(2), 274-283.e5.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!