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Phospho erk 1 2 9101

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Phospho-ERK 1/2 (9101) is a primary antibody that specifically binds to the phosphorylated forms of Extracellular Signal-regulated Kinase 1/2 (ERK1/2). It is used to detect and quantify the activation of the Ras/MAPK signaling pathway.

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Lab products found in correlation

Rhbmp2, by Thermo Fisher Scientific (1 mentions) Plx4032 vemurafenib, by Selleck Chemicals (1 mentions) Brain derived neurotrophic factor (bdnf), by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions) Epidermal growth factor (egf), by ProSpec (1 mentions) Egfr d38b1, by Cell Signaling Technology (1 mentions)

2 protocols using phospho erk 1 2 9101

1

Molecular Pathway Activation Analysis

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Reagents were obtained from the following sources: BDNF from PeproTech, Rocky Hill, NJ (450–02); rhBMP-2 from Life Technologies Gibco®. PLX4032 (Vemurafenib) from Selleckchem; EGF from ProSpec; NGF (mouse NGF 2.5S) from Thermo Fisher Scientific, Waltham, MA. Antibodies were obtained from the following sources: Alexa Fluor 568 conjugated secondary from Life Technologies; β-Actin from Sigma-Aldrich; HRP-Conjugated mouse (31430) and rabbit (31460) secondary from Pierce Thermo Scientific; total-Smad 1/5/8 (sc-6031), total-EGFR (SC03), and phospho-EGFR (S1046) (sc-101665) from Santa Cruz Biotechnology, Dallas, TX; Myc, phospho-Smad 1/5/8 (13820), phospho-EGFR (Y1068), phospho-Raf (Ser259) (9421), total-ERK1/2 (9102), and phospho-ERK 1/2 (9101) from Cell Signaling Technology, Danvers, MA.
Cruz E., Kumar S., Yuan L., Arikkath J, & Batra S.K. (2018). Intracellular amyloid beta expression leads to dysregulation of the mitogen-activated protein kinase and bone morphogenetic protein-2 signaling axis. PLoS ONE, 13(2), e0191696.
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2

Quantifying EGFR and ERK1/2 Phosphorylation

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Western blot analysis of EGFR and ERK1/2 phosphorylation was done as previously described [9 (link)]. The following primary antibodies were used: rabbit monoclonal Ab EGFR #D38B1 (1:1000), rabbit polyclonal phospho-EGFR #2234, rabbit polyclonal ERK1/2 #9102 (1:1000), and rabbit polyclonal phospho-ERK1/2 #9101 (all Cell Signalling, Leiden, The Netherlands). Protein bands were quantified by densitometry using ImageJ software (National Institutes of Health, Bethesda, MD, USA).
Amatngalim G.D., Broekman W., Daniel N.M., van der Vlugt L.E., van Schadewijk A., Taube C, & Hiemstra P.S. (2016). Cigarette Smoke Modulates Repair and Innate Immunity following Injury to Airway Epithelial Cells. PLoS ONE, 11(11), e0166255.
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