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Tetrazine peg5 nhs ester

Manufactured by Vector Laboratories
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Tetrazine-PEG5-NHS ester is a bifunctional linker molecule used in bioconjugation applications. It contains a tetrazine functional group for rapid inverse-electron-demand Diels-Alder reactions and an NHS ester for covalent attachment to primary amines. The PEG5 spacer provides water solubility and flexibility.

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Lab products found in correlation

Trans cyclooctene nhs ester, by Vector Laboratories (2 mentions) Mabthera, by Roche (2 mentions) Irdye800cw carboxylic acid, by LI COR (1 mentions) Sulfo cyanine5 nhs ester, by Lumiprobe (1 mentions) Sulfo cyanine7 carboxylic acid, by Lumiprobe (1 mentions) Protein a column, by Thermo Fisher Scientific (1 mentions) Tco peg4 nhs ester, by Vector Laboratories (1 mentions)

3 protocols using tetrazine peg5 nhs ester

1

Bioconjugation of Rituximab with Fluorescent Dyes

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General. All chemicals and solvents were obtained as reagent grade from commercial sources unless otherwise stated. Trans-Cyclooctene-NHS ester and Tetrazine-PEG5-NHS ester were purchased from Click Chemistry Tools (Scottsdale, AZ, USA). Rituximab (MabThera®, Roche Pharma AG, Grenzach-Wyhlen, Germany) was a kind gift from the University Hospital of Innsbruck. The fluorescent dyes, SulfoCyanine5-NHS ester and SulfoCyanine7 carboxylic acid were obtained from Lumiprobe GmbH (Hannover, Germany) while IRDye800CW carboxylic acid was purchased from LI-COR Biosciences GmbH (Bad Homburg, Germany).
Summer D., Petrik M., Mayr S., Hermann M., Kaeopookum P., Pfister J., Klingler M., Rangger C., Haas H, & Decristoforo C. (2020). Hybrid Imaging Agents for Pretargeting Applications Based on Fusarinine C—Proof of Concept. Molecules, 25(9), 2123.
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2

Bioorthogonal Chemical Conjugation

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All chemicals and solvents were purchased as reagent grade from commercial sources unless otherwise stated. trans-Cyclooctene-NHS ester and tetrazine-PEG5-NHS ester were bought from Click Chemistry Tools (Scottsdale, AZ, USA). Rituximab (MabThera®, Roche Pharma AG, Grenzach-Wyhlen, Germany) was of pharmaceutical grade and was a kind gift from the University Hospital of Innsbruck.
Summer D., Mayr S., Petrik M., Rangger C., Schoeler K., Vieider L., Matuszczak B, & Decristoforo C. (2018). Pretargeted Imaging with Gallium-68—Improving the Binding Capability by Increasing the Number of Tetrazine Motifs. Pharmaceuticals, 11(4), 102.
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3

Bioconjugation of TRC105 and ALT-836

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TRC105 and ALT-836 were respectively provided by TRACON Pharmaceuticals Inc. (San Diego, CA, USA) and Altor Bioscience Corporation (Miramar, FL, USA). 2-S-(4-isothiocyanatobenzyl)-1,4,7-triazacyclononane-1,4,7-triacetic acid (p-SCN-Bn-NOTA) was obtained from Marocyclics, Inc. (Dallas, TX, USA). TCO-PEG4-NHS ester and tetrazine-PEG5-NHS ester were purchased from Click Chemistry Tools (Scottsdale, AZ, USA). Pierce immobilized papain, protein A column, and all other reaction buffers and chemicals were purchased from Thermo Fisher Scientific (Carlsbad, CA, USA).
Luo H., England C.G., Shi S., Graves S.A., Hernandez R., Liu B., Theuer C.P., Wong H.C., Nickles R.J, & Cai W. (2016). Dual-targeting of tissue factor and CD105 for preclinical PET imaging of pancreatic cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(15), 3821-3830.
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