Rabbit anti-Neuroligin-1 and anti-Neuroligin-3 were previously described53 (link). Rabbit anti-GluR2/3 was a kind gift from Dr.Nathalie Sans. The following commercially available antibodies were used: rabbit anti-vesicular glutamate transporter 1 (vGluT1), rabbit anti-vesicular GABA transporter (vGAT), mouse anti-Vesicle associated membrane protein 2 (VAMP2), rabbit anti-Homer1, mouse anti-gephyrin, mouse anti-NR1 (Synaptic Systems), rabbit anti-GAPDH (Enogene), mouse anti-actin (Sigma), sheep anti-parvalbumin (PV) (R&D), goat anti-calretinin (CR) (Swant), mouse anti-GAD67, anti-NR2A (Upstate Biotechnology), mouse anti-NR2B, mouse anti-PSD95, mouse anti-GABAβ3, mouse anti-Kv3.1b (Neuromab), mouse anti-GAD65 (Developmental Studies Hybridoma Bank), mouse anti-MAP2 (Chemicon), rabbit anti-NeuN (Novus Biologicals), mouse anti-CaMKII (ABR Affinity BioReagents), rabbit anti-acetylhistone3 (Cell Signalling).
Secondary antibodies with minimal interspecies cross-reactivity conjugated to cyanine and Alexa 633, 546 or 488 dyes (Jackson ImmunoResearch and Invitrogen) were used for visualization in immunostaining. Secondary HRP conjugated anti-mouse and anti-rabbit IgG, and IRDye 680 coupled anti-Mouse and IRDye 800 coupled anti-Rabbit IgG for quantitative western blotting were purchased from Jackson and LI-COR Biosciences, respectively.
Secondary antibodies with minimal interspecies cross-reactivity conjugated to cyanine and Alexa 633, 546 or 488 dyes (Jackson ImmunoResearch and Invitrogen) were used for visualization in immunostaining. Secondary HRP conjugated anti-mouse and anti-rabbit IgG, and IRDye 680 coupled anti-Mouse and IRDye 800 coupled anti-Rabbit IgG for quantitative western blotting were purchased from Jackson and LI-COR Biosciences, respectively.