Magna RIPTM RNA kit (Millipore) was utilized to carry out RIP assay in conformity with the instructions of manufacturer. Transfected cells were lysed by RIP lysis buffer, and cell extracts were incubated with magnetic beads coated with Ago2 antibody (Millipore) or negative control IgG (Millipore). Afterwards, the beads were washed and treated with Proteinase K to digest proteins. The immunoprecipitated RNA was collected, purified and determined by RT‐qPCR assay.
Magna riptm rna kit
Manufactured by Merck Group
Sourced in United States
The Magna RIPTM RNA kit is a laboratory equipment product designed to efficiently extract and purify RNA from various sample types. It utilizes magnetic bead-based technology to capture and isolate RNA molecules, providing a reliable and reproducible method for RNA extraction.
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Lab products found in correlation
6 protocols using magna riptm rna kit
1
Ago2-RIP Assay Protocol
2
Analyzing Ago2-Mediated RNA Interactions
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RIP was performed using a Magna RIPTM RNA kit (Millipore, USA). Briefly, cultured chondrocytes were suspended in RIP lysis buffer (Solarbio) and incubated in RIP buffer containing human anti-Ago2 antibody beads (Millipore) overnight (Input and normal IgG served as controls). Next, RNAs were extracted using TRIzol reagent to follow the relative enrichment of MEG3/FOXO1 and miR-361-5p.
3
Immunoprecipitation of RNA-Binding Proteins
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RIP assay was performed using the Magna RIPTM RNA kit (Millipore) according to the previous study [41 (link)]. Cells were lysed in lysis buffer containing protease inhibitor cocktail and RNase inhibitor. Cells were incubated with RIP buffer containing magnetic beads coated with Ago2 antibody for 2 h at 4°C (Millipore). The antibody IgG was used as a negative control. After incubation, the coprecipitated RNA was eluted from beads and subjected to RT-qPCR analysis.
4
RNA Immunoprecipitation Assay Protocol
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Magna RIPTM RNA kit (Millipore) was utilized to conduct RNA immunoprecipitation (RIP) assay in line with the supplier’s protocol. Transfected MKN45 or AGS cells at a confluence of 80–90% were lysed with RIPA plus protease inhibitor and RNA enzyme inhibitor. IgG, Ago2, and eIF4G antibodies (Abcam) were separately added and incubated with cell extraction. After digesting proteins, immunoprecipitated RNA was collected and subjected to qRT–PCR after purification.
5
RIP Analysis of LINC00485/Twist1 and miR-361-5p
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Using a Magna RIPTM RNA kit (Millipore, USA), RIP analysis was carried out. Cultured chondrocytes were suspended in RIP lysis buffer and were further incubated overnight in RIP buffer that contained human anti-Ago2 antibody beads (Millipore) (Input and normal IgG served as controls). Afterward, RNA extraction was achieved using the TRIzol reagent and the relative enrichment of LINC00485/Twist1 and miR-361-5p was tested via the RT-PCR analysis.
6
RNA-Immunoprecipitation Assay Protocol
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The Magna RIPTM RNA kit (Millipore, Bedford, MA, USA) was used for RNA-immunoprecipitation (RIP) assay. In brief, the treated cells were lysed with RIPA solution supplement with protease inhibitor and RNA enzyme inhibitor. Cell extraction was incubated with IgG and MS2 antibodies. Protein samples were digested, and immuno-precipitated RNA was harvested. The expression level of purified RNA was detected by RT-qPCR.
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