Three groups of MCF-7 cells (4,000 cells/well) in the logarithmic growth phase were inoculated into 96-well plates. After the cells adhered to the wall, various chemotherapeutic drugs were added. The final concentrations of docetaxel (Beijing Solarbio Science & Technology Co., Ltd.) were 0.2,0.4, 0.8, 1.0 and 1.2 µmol/l and the final concentrations of cis-platinum (Beijing Solarbio Science & Technology Co., Ltd.) complexes were 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 µmol/l. Three replicate wells were set at each concentration. Cells in the B.C group were used as control wells. Blank wells without any cells were filled with equal volume (100 µl) of DMEM (cat. no. 01-052-1ACS; Biological Industries). After 24 h of drug treatment, Cell Counting Kit (CCK)-8 (cat. no. ab228554, Abcam) reagent was added at 100 µl per well and mixed, followed by incubation for 1 h at room temperature according to the manufacturer's protocols. Finally, the optical density (OD) at 450 nm was determined for each well using an enzyme-linked immunosorbent assay detection system (HBS-1096c; Nanjing Detielab Experimental Equipment Co., Ltd.). Cell viability and the half maximal inhibitory concentration (IC50) of the two drugs in different groups were calculated using GraphPad Prism version 6 (GraphPad Software).
Docetaxel
Manufactured by Solarbio
Sourced in China
Docetaxel is a chemotherapy medication used to treat various types of cancer. It is a cytotoxic agent that inhibits cell division by disrupting the microtubule network. Docetaxel is commonly used in the treatment of breast, lung, prostate, and other solid tumors.
Automatically generated - may contain errors
Lab products found in correlation
Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions)
Cis platinum, by Solarbio (1 mentions)
Ab137852, by Abcam (1 mentions)
Ab119796, by Abcam (1 mentions)
Ab114977, by Abcam (1 mentions)
Ab7291, by Abcam (1 mentions)
α tubulin, by Abcam (1 mentions)
All in one first strand cdna synthesis kit, by GeneCopoeia (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
β actin, by Abcam (1 mentions)
All in one qpcr mix, by GeneCopoeia (1 mentions)
2 protocols using docetaxel
1
Comparative Cytotoxicity of Docetaxel and Cisplatin in MCF-7 Cells
2
Docetaxel and Lactate Metabolism Regulation
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Docetaxel (ID0400) and lactate (L8601) obtained from Solarbio life science (Beijing, China). Lactate assay kits (KA0833) was purchased from Abnova. The SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) (9003) was from Cell Signaling Technology (Danvers, MA, USA). Glucose uptake fluorometric assay kit (MAK084) was purchased from Sigma Aldrich (St. Louis, MO, USA). Antibodies targeting PKM2 (Abcam, ab137852,1:2000 for WB), HIF-1α (Abcam, ab114977,1:2000 for WB), PFKP (Abcam, ab119796,1:2000 for WB), α-tubulin (Abcam, ab7291,1:4000 for WB) and β-actin (Abcam, ab8226,1:4000 for WB) were purchased from Abcam Company (MA, USA); Fetal boive serum (FBS), Alexa-488- and 594-conjugated secondary antibodies were from Invitrogen (Thermo Fisher Scientific). The trizol was purchased from Invitrogen and All-in-One First-Strand cDNA Synthesis Kit and All-in-One qPCR Mix were obtained from GeneCopoeia (Guangzhou, China). Subcellular fractionation was conducted using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (thermo fisher) following the manufacturer’s recommendations. All ultrapure reagents were from Promega (Madison, WI, USA).
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