Recipient estrus synchronization was initiated by inserting an intravaginal progesterone device (1.38 g; Eazi-Breed CIDR; Zoetis) and intramuscular administration of gonadotropin (100 mcg; Factrel; Zoetis) on day 0 (sixteen days prior to transfer). On day 7, the CIDR was removed and intramuscular prostaglandin (25 mg; Lutalyse; Zoetis) was administered. Recipients were monitored for estrus, and a second intramuscular dose of gonadotropin (100 mcg; Factrel; Zoetis) was administered on day 9. Prior to transfer on day 16, recipient response to synchronization was confirmed via detection of an appropriate corpus luteum with transrectal ultrasonography. Prior to transfer, each recipient received a caudal epidural using 100 mg 2% lidocaine (Xylocaine; Fresenius). Embryos were transferred via non-surgical, transcervical technique, and the blastocyst was deposited into the uterine horn ipsilateral to the corpus luteum. Pregnancy was diagnosed on day 35 of embryonic development by transrectal ultrasonography (5.0 MHz linear probe; EVO Ibex, E.I. Medical Imaging).
Factrel
Manufactured by Zoetis
Sourced in United States
Factrel is a pharmaceutical product manufactured by Zoetis. It contains the active ingredient gonadorelin, which is a synthetic version of the natural gonadotropin-releasing hormone (GnRH) found in animals. Factrel is used in veterinary medicine to stimulate the release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the pituitary gland in animals.
Automatically generated - may contain errors
Lab products found in correlation
Lutalyse, by Zoetis (12 mentions)
Eazi breed cidr, by Zoetis (6 mentions)
Xylocaine, by Fresenius (3 mentions)
Lutalyse sterile solution, by Zoetis (3 mentions)
Pgf2α, by Zoetis (2 mentions)
Lutalyse highcon, by Zoetis (2 mentions)
Ssd 500v, by Aloka (2 mentions)
Eazi breed cidr cattle insert, by Zoetis (2 mentions)
Lps escherichia coli o55 b5, by Merck Group (1 mentions)
Eazi breed, by Zoetis (1 mentions)
Chorulon, by Merck Group (1 mentions)
Estrumate, by Merck Group (1 mentions)
Dinoprost tromethamine, by Zoetis (1 mentions)
Prostaglandin f2α, by Zoetis (1 mentions)
Wnt3a, by R&D Systems (1 mentions)
20 protocols using factrel
1
Synchronizing Estrus for Embryo Transfer
2
Bovine Embryo Transfer via Estrus Synchronization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Estrus synchronization of recipient cattle began 16 days prior to the embryo transfer with the use of an intravaginal progesterone releasing device (1.38 g; Eazi-Breed CIDR; Zoetis) and the administration of gonadorelin (100 mcg; Factrel; Zoetis) done on day 0. On day 7, the CIDR was removed and prostaglandin (25 mg; Lutalyse; Zoetis) was administered. A second dose of gonadorelin (100 mcg; Factrel; Zoetis) was given on day 9 and recipients were monitored for signs of estrus. Confirmation of recipient synchronization was done on day 15 via corpus luteum detection using a transrectal ultrasound. On day 16, embryo transfers were performed. Recipients received a caudal epidural of 100 mg 2% lidocaine (Xylocaine; Fresenius) prior to embryo transfer. Two to three blastocysts were loaded into 0.25 cc straws and transferred using the non-surgical transcervical technique into the uterine horn ipsilateral to the corpus luteum. On day 35 of gestation, transrectal ultrasonography (5.0 MHz linear probe; EVO Ibex, E.I. Medical Imaging) was done to confirm pregnancies, and reconfirmed on day 80. A total of four embryo transfers were performed, and recipients were resynchronized for subsequent embryo transfers if they did not become pregnant from prior embryo transfers.
3
Estrus Synchronization for Embryo Transfer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recipient estrus synchronization was initiated by inserting an intravaginal progesterone device (1.38g; Eazi-Breed CIDR; Zoetis) and intramuscular administration of gonadotropin (100mcg; Factrel; Zoetis) on day 0 (sixteen days prior to transfer). On day 7, the CIDR was removed and intramuscular prostaglandin (25mg; Lutalyse; Zoetis) was administered. Recipients were monitored for estrus, and a second intramuscular dose of gonadotropin (100mcg; Factrel; Zoetis) was administered on day 9. Prior to transfer on day 16, recipient response to synchronization was confirmed via detection of an appropriate corpus luteum with transrectal ultrasonography. Prior to transfer, each recipient received a caudal epidural using 100mg 2% lidocaine (Xylocaine; Fresenius). Embryos were transferred via non-surgical, transcervical technique, and the blastocyst was deposited into the uterine horn ipsilateral to the corpus luteum.
Pregnancy was diagnosed on day 35 of embryonic development by transrectal ultrasonography (5.0 MHz linear probe; EVO Ibex, E.I. Medical Imaging).
Pregnancy was diagnosed on day 35 of embryonic development by transrectal ultrasonography (5.0 MHz linear probe; EVO Ibex, E.I. Medical Imaging).
4
Somatotropin Effects on Follicular Dynamics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The study was performed with non-lactating Jersey dairy cows (n=21), kept in pasture. The cows had body condition score (BCS) between 2.5 and 3.5 (on a scale of 1 to 5) and were randomly (by BCS) assigned to two treatments: control (n=10) and somatotropin (rbST, n=11). The rbST group received one injection of somatotropin (500 mg/cow, s.c., Lactotropin®, Elanco, SP, Brazil) 60 days before the beginning of synchronization protocol (Day -60). On day 0, all cows received an intravaginal progesterone (P4)-releasing device (1.9 g, CIDR®, Zoetis, NJ, USA) and a GnRH injection (100 mg, IM, Factrel®, Zoetis). On Day 8, at the moment of P4 device withdrawal, all cows received an injection of PGF2α (25 mg, i.m., Lutalyse®, Zoetis). Transrectal ultrasonography (7.5 MHz linear array probe, Aquila pro, Esaote, São Paulo, SP, Brazil) was performed on days -60, -53, -46, -14, -7, 0 and 8 for antral follicle count. All follicles detected by ultrasonography were counted and classified according to its diameter in three categories: Class 1 (<6 mm), Class 2 (6-9 mm) and Class 3 (>9 mm). On day 8 of the protocol, the diameter of the largest follicle was measured through ultrasonography.
5
Reproductive Management of Primiparous Cows
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For experiment 1, weekly cohorts of primiparous cows were enrolled in the study at 60 ± 3 DIM (study d 0). Cows (n = 1,358) were assigned randomly based on ear tag identification number to 2 treatments: (1) treatment with 100 μg of GnRH (Factrel, Zoetis Inc., Florham Park, NJ; Gpresynch), or (2) no treatment (control) at study d 0. At study d 7, all cows were presynchronized with two 25-mg injections of PGF 2α (dinoprost tromethamine; 5 mL of Lutalyse, Zoetis Inc.) injections given 14 d apart. Estrus detection was performed once daily based on tail paint removal. Cows detected in estrus were eligible to be inseminated after 53 DIM. Once inseminated, cows received no further injections of the synchronization protocol. Cows not inseminated by study d 35 were enrolled in the Cosynch-72 protocol for TAI (Figure 1).
6
Immune Response of Heifers to LPS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All procedures were approved by the New Mexico State University Institutional Animal Care and Use Committee (2017–030). Fourteen crossbred heifers (BW average = 416 kg) were housed and maintained at New Mexico State University (Las Cruces, NM).
Heifers were stratified by BW and assigned to vehicle-treated control (CON, n = 7) or LPS (n = 7) treatment groups. LPS (Escherichia coli O55:B5; Sigma–Aldrich, Inc., Milwaukee, WI) was prepared by dissolving 1 mg in 5 mL of sterile saline, then subcutaneously injected at a rate of 2.0 μg/kg of BW. Each animal received approximately 4 mL of solution on days 2, 5, and 8. Control animals were injected with sterile saline. Baseline rectal temperatures were taken 1 d before the start of the experiment to preclude any potential febrile response prior to administering treatment.
Heifers were synchronized to estrus using the Select Synch plus controlled internal drug release (CIDR ) protocol. On day 0, a GnRH (2 mL, i.m., Factrel, Zoetis Inc. US) injection was administered, and a CIDR (Eazi-Breed, Zoetis Inc., Kalamazoo, MI) device was inserted. On day 7, the CIDR was removed, and heifers received PGF2á (Lutalyse, 25 mg, i.m., Zoetis Inc. US).
Rectal temperatures were taken just prior to treatment (30-Second Digital Thermometer, Target Corporation, Minneapolis, MN) and at 2, 3, 4, 8, and 10 h after injection.
Heifers were stratified by BW and assigned to vehicle-treated control (CON, n = 7) or LPS (n = 7) treatment groups. LPS (Escherichia coli O55:B5; Sigma–Aldrich, Inc., Milwaukee, WI) was prepared by dissolving 1 mg in 5 mL of sterile saline, then subcutaneously injected at a rate of 2.0 μg/kg of BW. Each animal received approximately 4 mL of solution on days 2, 5, and 8. Control animals were injected with sterile saline. Baseline rectal temperatures were taken 1 d before the start of the experiment to preclude any potential febrile response prior to administering treatment.
Heifers were synchronized to estrus using the Select Synch plus controlled internal drug release (
Rectal temperatures were taken just prior to treatment (30-Second Digital Thermometer, Target Corporation, Minneapolis, MN) and at 2, 3, 4, 8, and 10 h after injection.
7
Synchronization and hCG Treatments in Dairy Cows
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cows between 39 and 64 DIM (mean ± SD = 45.2 ± 6.0) were submitted to a pretreatment for synchronization of ovulation, consisting of an Ovsynch + controlled internal drug-release insert (CIDR; Figure 1; Pursley et al., 1995) (link). Briefly, cows received GnRH (200 µg i.m.; Factrel, Zoetis) and a P4 intravaginal insert (Eazi-Breed CIDR; Zoetis). Seven days later, the P4 insert was removed, and prostaglandin F 2α (PGF 2α 25 mg i.m.; Lutalyse HighCon, Zoetis) was administered, followed by another PGF 2α 1 d later and a second GnRH 1 d after the second PGF 2α . Only synchronized cows with complete CL regression 2 d after PGF 2α (serum P4 < 1.0 ng/mL) and ovulation by 2 d after the last GnRH were used in the study (n = 64). The last GnRH of pretreatment was considered d 0 of the estrous cycle. After confirmation of synchronization, cows were randomly assigned to one of 3 treatments. Control cows (n = 22) did not receive any treatment except the synchronization protocol. Cows enrolled in the second treatment group (hCG7; n = 20) received an i.m. treatment with 3,300 IU of hCG (Chorulon, Merck Animal Health) 7 d after the last GnRH (d 7 of the cycle), and cows enrolled in the third treatment group (hCG7+13; n = 22) received 2 hCG treatments with 3,300 IU, one on d 7 and a second on d 13 after the last GnRH (d 7 and d 13 of the estrous cycle).
8
Synchronizing Estrus in Dairy Cows
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To insure the protocol (summarized in Figure 1 ) started at 59 ± 3 DIM but did not begin with the first cycle of the cow, all cows received a vaginal implant containing 1.38 g of P4 (P4; CIDR, Zoetis, Kirkland, QC, Canada) and an intramuscular (i.m.) injection of 100 μg of gonadorelin (GnRH; Factrel, Zoetis, Kirkland, QC, Canada; 2 ml) at enrolment. After 7 days, the implant was removed, and cows received an i.m. injection of 500 μg of cloprostenol (PGF2α; Estrumate, Merck Animal Health, Kirkland, QC, Canada; 2 ml). The protocol started (0 days) 2 days later, when all cows received a second injection of GnRH (as above). Cows with a short length LP received an injection of PGF2α (as above) on 7 days. All cows then received an injection of GnRH on 9 days, two injections of PGF2α 12 h apart on 14 days, and finally 0.5 mg of estradiol cypionate i.m. (ECP; Estrus, Rafter8, Calgary, AB, Canada; 0.5 ml) on 15 days, 12 h after the last PGF2α. With these protocols, the LP preceding the estrus in the short and normal length LP were 5 and 14 days, respectively.
9
Double-Ovsynch Protocol for Estrous Synchronization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cows had their estrous cycles synchronized for first TAI using a Double-Ovsynch protocol starting at 53 ± 3 DIM in herd A and 51 ± 3 DIM in herd B, as described by Souza et al. (2008) and later modified by Brusveen et al. (2009) . The GnRH (100 μg/dose of gonadorelin hydrochloride, Factrel) and the PGF 2α (25 mg/dose of dinoprost tromethamine, Lutalyse) were from Zoetis (Madison, NJ). Briefly, cows received the first GnRH treatment of the Presynch portion of the Double-Ovsynch, followed by a treatment with PGF 2α 7 d later and GnRH 72 h after PGF 2α . Seven days later, cows received a GnRH (G1) treatment followed by 2 PGF 2α treatments administered 7 and 8 d later, with the last GnRH (G2) treatment administered 56 h after the first PGF 2α treatment and AI 16 to 20 h later (Figure 1). Within each herd, cows (n = 800; n = 560 herd A; n = 240 herd B) were blocked by parity (primiparous vs. multiparous) and randomly assigned to serve as untreated controls (high-P4) or to receive a half dose of PGF 2α (low-P4; 12.5 mg of dinoprost tromethamine) 2 d before G1 (Figure 1). At each location, multiple sires with high genetic merit and proven fertility were used and were equally balanced between treatments.
10
Breeding and Pregnancy Outcomes in Lactating Cows
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
This experiment had the same objective as in experiment 1, but it was conducted using lactating cows. From 06/2020 to 09/2020, Holstein dairy cows (first service, 60–70 days in milk, 2nd-5th parity) were synchronized to estrus via intramuscular injections of PGF2A (Lutalyse, 25 mg dinoprost tromethanmine, Zoetis) and gonadotropin releasing hormone (GnRH) (Factrel, 100 μg gonadorelin, Zoetis) following the PG-3-G Ovsynch (Peters and Pursley, 2002 (link)). Cows were assigned randomly to be either inseminated (N = 8) or not inseminated (N = 6) with blood collection on the same days as in experiment 1. Pregnancy was confirmed on day 30 after insemination using transrectal ultrasonography. All pregnant animals delivered healthy calves.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!