Anti il 10

Immunohistochemistry staining of CD19, IL-10, and C-X-C motif chemokine 13 (CXCL13) in kidney tissues was performed, respectively. Briefly, 4 μm sections obtained from formalin-fixed, paraffin-embedded kidney tissue samples were incubated with 1:500-diluted anti-CD19, 1:1000-diluted anti-CXCL13 (rabbit anti-human; Abcam, Cambridge, UK), and 1:500-diluted anti-IL-10 (rabbit anti-human; Absin, Shanghai, China) primary antibodies overnight at 4°C, followed by Horseradish Peroxidase-conjugated 1:20,000-diluted goat anti-rabbit secondary antibody (Abcam, Cambridge, UK) for 1 h at room temperature and then 3,3′-diaminobenzidine for another 10 min. DAPI appears in blue. Immunohistochemistry images were acquired with an Aperio ScanScope AT Turbo (Aperio, Vista, CA). Numbers of CD19, IL-10, and CXCL13 positivities were scored as follows: 0 positivity, score = zero; 1–5 positivities, score = one; 6–10 positivities, score = two; 11–20 positivities, score = three; >20 positivities, score = four.

The Western Blotting Substrate Kit (32132) was purchased from Thermo Fisher Scientific™. The bicinchoninic acid (BCA) protein assay kit (23227) was purchased from Pierce™. The TRIzol reagents were purchased from Life Technologies. The Revert Aid Reverse Transcriptase was purchased from Thermo Fisher Scientific™. The TB Green™ Premix Ex Taq™ was purchased from Takara. anti‐IL‐10 (Cat. abs128784), anti‐JAK2 (Cat. abs118049), anti‐STAT1 (Cat. abs115001), anti‐Hsp90 (Cat. abs130560a), anti‐phospho‐JAK2 (Cat. abs139986a) and anti‐phospho‐STAT1 (Cat. abs130924a) were purchased from Absin Bioscience Inc. anti β‐Actin(Cat. CW0096) was purchased from Beijing Comwin Biotech Co., Ltd.