The mouse luminal epithelial cells were isolated as previously described (26 (link)). Briefly, the uteri were digested with sterile HBSS containing 0.3% trypsin and 6 mg/ml dispase for 1.5 h at 4 °C followed by 30 min at room temperature and 10 min at 37 °C. The luminal epithelial cells were cultured in DMEM/F-12 medium (Sigma-Aldrich) containing 10% heat-inactivated fetal bovine serum (FBS, Invitrogen, USA). The epithelial cells were treated with recombinant HMGB1 (Sigma-Aldrich).
Recombinant hmgb1
Manufactured by Merck Group
Sourced in United States
Recombinant HMGB1 is a laboratory reagent produced by Merck Group. It is a purified recombinant form of the High Mobility Group Box 1 (HMGB1) protein. HMGB1 is a nuclear protein involved in DNA-related cellular processes.
Automatically generated - may contain errors
2 protocols using recombinant hmgb1
1
Isolation and Culture of Mouse Luminal Epithelial Cells
2
Virus Studies with Doxorubicin and HMGB1
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Doxorubicin and Glycyrrhizin were resuspended in DMSO (all Sigma-Aldrich, St. Louis, MO, USA). Recombinant HMGB1 (Sigma-Aldrich, St. Louis, MO, USA) was resuspended in PBS. HSV1716gfp was used for virus studies as described previously [36 (link)]. In brief, HSV1716gfp is derived from HSV strain 17+ with deletions of both copies of the RL1 gene encoding for the neurovirulence factor ICP34.5 (HSV1716). Green fluorescent protein (GFP) is added to the RL1 gene locus and driven by the phosphoglycerate kinase (PGK) promoter [37 (link)].
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