Am251 n piperidin 1 yl 5 4 iodophenyl 1 2 4 dichloro phenyl 4 methyl 1h pyrazole 3 carboxa mide

We purchased BAPTA (1,2-Bis(2-Aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid) from Sigma (St. Louis, MO), CGP 55845A, DL-AP5 and DNQX from Tocris (Ellisville, MO), ethanol from Remet (La Mirada, CA) and TTX from Biotium (Hayward, CA). WIN (WIN55,212-2; [(3R)-2,3-dihydro-5-methyl-3-(4-morpholinylmethyl) pyrrolo [1,2,3-de]-1,4-benzoxazin-6-yl]-1-naphthalenyl-methanone, monomethanesulfonate) and AM251 (N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-2,4-dichloro phenyl)-4-methyl-1H-pyrazole-3-carboxa-mide) were purchased from Cayman Chemical (Ann Arbor, MI), and dissolved in dimethylsulfoxide (final concentrations of 0.05–0.1%), which had no effect on synaptic responses in control experiments.

Paraoxon (PO) and chlorpyrifos oxon (CPO were purchased from ChemService (West Chester, PA; both >98% purity). [³H]anandamide (ethanolamine-1-³H), specific activity 60 Ci/mmol) was purchased from American Radiochemical Company (St. Louis, MO). Arachidonoyl-1-thio-glycerol, anandamide, and AM 251 (N-(piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide) were purchased from Cayman Chemical (Ann Arbor, MI). Acetylthiocholine iodide and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO).