Harvested organs (brain, liver, lungs, kidney, and spleen) were snap frozen. All organ tissues were postfixed in 4% PFA for 48 h. Five μm sections were obtained and stained with hematoxylin (H) and eosin (E). The H&E stained sections were scanned and the images were captured using the Olympus VS120 image analysis system (Olympus America Inc., Philadelphia, PA).
Vs120 image analysis system
Manufactured by Olympus
The VS120 image analysis system is a comprehensive imaging solution designed for a wide range of applications. It enables high-quality image acquisition, analysis, and visualization of samples across various fields of study.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using vs120 image analysis system
1
Histological Analysis of Harvested Organs
2
Bone Microstructure Imaging and Analysis
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Soft tissues were removed from left tibiae and lumbar 1 (L1) vertebrae, fixed in 10% formalin at 4 °C for 24–48 hours on a rocker and transferred to 70% ethanol until μCT scanning. Specimens were scanned at high resolution (10.5 μm) on a VivaCT 40 μCT scanner (Scanco Medical) using 300 ms integration time, 55 kVp energy and 145 μA intensity. Then these tibiae were decalcified in 10% EDTA at 4 °C for 21 days after formalin fixation and embedded in paraffin. Sections (4 μm thick) were stained with H&E for bone volume and osteoblast (OB) analysis and with TRAP-Fast Green for osteoclast (OC) analysis using OsteoMeasure system. OB and OC numbers and surfaces per millimeter bone surface were analyzed in tibial sections with point counting using an eyepiece grid and an Olympus TH4-100 microscope. Tibial and vertebral bone volumes were analyzed in digital slides generated using an Olympus VS120 Image Analysis System and automated analysis and algorithms that we developed using a Visiopharm Image Analysis System52 (link).
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