MNCs or CD38+ NK cells were incubated in media containing 50 μM C3G or an equal volume of PBS at 37 °C for 24 h. CD38+ NK cells were then washed twice and cocultured with CD38+ NK-depleted MNCs in a transwell apparatus at 37 °C and 5% CO2 for 48 h. The MNCs or CD38+ NK-depleted MNCs were incubated with PerCP anti-CD4 antibody (BioLegend) and APC anti-CD25 antibody (BioLegend) for 30 min. After washing with PBS and centrifugation, binding buffer was added to resuspend the cell pellet. FITC anti-Annexin V antibody (BioLegend) was added to the cells, and they were incubated in the dark for 15 min. The percentage of annexin V+ CD4+ CD25+ Treg cells in the CD4+ CD25 Tregs was quantified by flow cytometry using routine methods.
Fitc anti annexin 5 antibody
Manufactured by BioLegend
FITC anti-Annexin V antibody is a fluorescently labeled antibody that binds to Annexin V, a protein that has a high affinity for phosphatidylserine. This antibody can be used to detect and quantify cells undergoing apoptosis (programmed cell death).
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using fitc anti annexin 5 antibody
1
Treg Induction by C3G in NK Cells
2
Apoptosis Assessment by Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were harvested and labelled with FITC anti-Annexin V antibody (Biolegend) and propidium iodine (PI) (Biolegend). After staining for 15 minutes in dark, cell death was assessed by flow cytometry (FACScalibur). Early apoptotic cells were positive for Annexin V and negative for PI whereas late apoptotic or early necrotic cells were positive for both Annexin V and PI.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!