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Heparin coated tubes

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Heparin-coated tubes are laboratory equipment used for the collection and storage of blood samples. The tubes are coated with heparin, an anticoagulant, to prevent the blood from clotting during the sampling process.

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Lab products found in correlation

Rnalater, by Thermo Fisher Scientific (2 mentions) Sepmate 50 tube, by STEMCELL (1 mentions) Ficoll, by GE Healthcare (1 mentions) Multiplate analyzer, by Roche (1 mentions) Paxgene blood rna tube, by BD (1 mentions) Ez dna methylation kit, by Zymo Research (1 mentions) Pyro q cpg software, by Qiagen (1 mentions) Penicillin streptavidin, by Merck Group (1 mentions) Rpmi 1640, by Merck Group (1 mentions) 24 well plate, by Sarstedt (1 mentions) Lymphoprep, by Axis-Shield (1 mentions)

Spelling variants of this product

Heparin (64 citations) Heparin tubes (39 citations) Lithium heparin coated tubes (8 citations) Sodium heparin-coated tubes (4 citations) Heparin-coated vacutainer tubes (3 citations) Sodium heparin-coated collection tubes (3 citations) Lithium heparin-coated microcentrifuge tubes (2 citations) Lithium heparin coated plasma separator tubes (2 citations) Lithium heparin-coated vacutainer tubes (2 citations) Lithium-heparin coated blood tubes (2 citations)

12 protocols using heparin coated tubes

1

PBMC Isolation from Whole Blood

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Healthy donors were recruited from the University of Otago. Collection of blood was approved by the Human Ethics Committee of the University of Otago and healthy donors consented under ethics number H18/088.
Whole blood was collected from donors in heparin coated tubes (Becton Dickinson, Franklin Lakes, NJ) and diluted at a 1:1 ratio with sterile PBS. Whole blood was layered over Ficoll (GE Healthcare) in SepMate‐50 tubes (StemCell Technologies, Vancouver, BC, Canada) according to the manufacturer's instructions. Cells were centrifuged at 1200 g for 10 mins at 4 °C. Supernatant with enriched peripheral blood mononuclear cells (PBMCs) were washed twice in PBS and centrifuged twice at 382 g for 4 mins. Cells were cryopreserved until use in the assay.
Major G., Longoni A., Simcock J., Magon N.J., Harte J., Bathish B., Kemp R., Woodfield T, & Lim K.S. (2023). Clinical Applicability of Visible Light‐Mediated Cross‐linking for Structural Soft Tissue Reconstruction. Advanced Science, 10(26), 2300538.
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2

Platelet Aggregation Dynamics in Sickle Cell

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Whole blood multi-electrode aggregometry (MEA) was performed using the Multiplate® analyzer (Roche Diagnostics) as previously described [30 (link)]. For steady state measurements, groups of AA, SS, SSCKO, and CKO mice were anesthetized with isoflurane and blood was collected from the vena cava into heparin-coated tubes (Becton Dickinson). MEA, in 300 µL of whole blood from each mouse, was performed over a 6-minute window following PAR4-TRAP (650 µM) agonist stimulation. Results reported as aggregation units (AU) are shown as area under the curve. A different group of AA, SS, and SSCKO mice was exposed to hypoxia/reoxygenation as described above, prior to platelet aggregation testing. Hypoxia/reoxygenation and aggregation testing were performed on the same group of CKO mice after one week of steady state testing.
Nwankwo J.O., Gremmel T., Gerrits A.J., Mithila F.J., Warburton R.R., Hill N.S., Lu Y., Richey L.J., Jakubowski J.A., Frelinger AL I.I.I, & Chishti A.H. (2017). Calpain-1 regulates platelet function in a humanized mouse model of sickle cell disease. Thrombosis research, 160, 58-65.
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3

Comprehensive Plasma Profiling Protocol

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After decapitation, blood was collected in heparin-coated tubes (Becton Dickinson, Plymouth, UK) and plasma isolated by centrifugation (20 min, 2200 x g, 4 °C) for analyses of plasma alanine aminotransferase activity (ALAT), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC) and lipoprotein cholesterol concentrations. Lipoproteins were separated into five fractions by single density gradient ultracentrifugation of plasma for 18h at 21 °C. 32 Plasma samples were stored at -80 °C until analysed, while cholesterol concentrations in lipoprotein fractions were determined immediately after ultracentrifugation. Erythrocytes were lysed by addition of an equal volume of MilliQ water and stored at -80 °C. One ml of blood was collected into a PAXgene blood RNA tube for purification of RNA from the white blood cells (Becton Dickinson, Plymouth, UK). For each analysis, all samples were run in a randomised order within the same batch to minimise analytical variation.
Ravn-Haren G., Krath B.N., Markowski J., Poulsen M., Hansen M., Kołodziejczyk K., Kosmala M, & Dragsted L.O. (2018). Apple pomace improves gut health in Fisher rats independent of seed content. Food & function, 9(5).
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4

Jejunum Specimens from Gastric Bypass Surgery

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Jejunum specimens were collected during laparoscopic Roux-En-Y gastric bypass surgery in Departments of Gastroenterology and Surgery, Lithuanian University of Health Sciences (Kaunas, Lithuania). Two cohorts of jejunum surgical samples from unrelated individuals were collected: cohort 1 (n = 56 individuals, 21–72 years old) and cohort 2 (n = 59 individuals, 22–65 years old) (Supplementary Fig. 1a). Following removal, jejunum samples were immediately submerged in RNAlater (Life Technologies), kept overnight at 4°C, followed by storage at −80°C. Blood (n = 58 individuals) and sperm (n = 18 individuals) samples were collected from unrelated individuals enrolled in laparoscopic surgery, and were from separate individuals than those in cohort 1 and 2. Blood samples were collected by venipuncture into heparin-coated tubes (BD), and then stored immediately at −80°C. Sperm samples provided by participants were stored immediately at −80°C. The study protocol was approved by the Bioethics Committee of Lithuanian University of Health Sciences (Protocol 2007-12-04 Nr.BE-2-55), and each patient signed informed consent to participate in the study. Human tissues were processed for mRNA analysis, DNA modification profiling and genotyping by researchers blind to any sample information.
Labrie V., Buske O.J., Oh E., Jeremian R., Ptak C., Gasiūnas G., Maleckas A., Petereit R., Žvirbliene A., Adamonis K., Kriukienė E., Koncevičius K., Gordevičius J., Nair A., Zhang A., Ebrahimi S., Oh G., Šikšnys V., Kupčinskas L., Brudno M, & Petronis A. (2016). Lactase non-persistence is directed by DNA variation-dependent epigenetic aging. Nature structural & molecular biology, 23(6), 566-573.
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5

Recruiting T1D Patients and Controls

Cited 1 time
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Control individuals were recruited from the general population at the KU LEUVEN (Leuven, Belgium). Patients with established T1D diagnosed on the basis of clinical criteria [24 (link)] were recruited from the clinical department of Endocrinology at the University Hospital Leuven. This study was approved by the institutional ethical board of the University hospital Leuven (S52697) and informed consent was obtained from every subject. Heparin-coated tubes (BD Biosciences, Erembodegem, Belgium) were used to collect peripheral blood for serum and peripheral blood mononuclear cells (PBMCs) isolation. The clinical characteristics for the patients have been summarized in S1 Table.
Korf H., Breser L., Van Hoeck J., Godoy J., Cook D.P., Stijlemans B., De Smidt E., Moyson C., Monteiro Carvalho Mori Cunha J.P., Rivero V., Gysemans C, & Mathieu C. (2017). MIF inhibition interferes with the inflammatory and T cell-stimulatory capacity of NOD macrophages and delays autoimmune diabetes onset. PLoS ONE, 12(11), e0187455.
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6

Plasma and PBMC Isolation Protocol

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Peripheral blood was collected in heparin-coated tubes (BD Biosciences, Franklin Lakes, NJ) and separated into plasma and peripheral blood mononuclear cells (PBMCs), which were stored at −80°C until analysis. Plasma and PBMCs from 46 age- and sex- matched HC were also used in the study.
Xia Y., Yang J., Sanyal A., Shah V., Chalasani N., Yu Q., Zheng X, & Li W. (2020). Persistent Hyperactivation of Endothelial Cells in Patients with Alcoholic Hepatitis. Alcoholism, clinical and experimental research, 44(5), 1075-1087.
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7

Peripheral Blood Cytokine and Cell Analysis

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Peripheral blood samples were collected from the subjects before any regional or systemic anticancer treatments and re-collected from 15 patients after thoracic surgery. The fresh peripheral blood of all individuals was stored in heparin-coated tubes (BD Biosciences, San Jose, CA, USA) and centrifuged at 4,000 rpm for 10 min at 4°C. Then, the cell-free supernatants, allocated into 1.5-ml Eppendorf tubes, were frozen at −80°C for the detection of cytokines (30 (link)). The cell pellets were re-suspended in saline for further analyses of flow cytometry and real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) (30 (link)).
Bao Z., Lu G., Cui D., Yao Y., Yang G, & Zhou J. (2016). IL-17A-producing T cells are associated with the progression of lung adenocarcinoma. Oncology Reports, 36(2), 641-650.
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8

Quantifying PTX3 Methylation in Esophagus

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Whole blood was collected in heparin coated tubes (BD Biosciences, San Jose, CA), centrifuged at 3,000× g for 10 mins, and plasma collected and stored at -80°C until analyzed. 100 μL of plasma from each rat was used to quantify PTX3 concentrations using an enzyme-linked immunosorbent assay (ELISA) (Cusabio, Carlsbad, CA). Pyrosequencing DNA was extracted from snap frozen esophageal epithelium (Qiagen) and standardized to 500 ng. It was then bisulfite converted using an EZ DNA Methylation Kit (Zymo Research, Orange, CA). A region within the PTX3 gene in each esophagus was amplified via PCR and the product was sequenced using a pyrosequencer (Qiagen). Gene methylation of PTX3 was quantified using PyroQ-CpG software (Qiagen).
Peiffer D.S., Zimmerman N.P., Wang L.S., Ransom B., Carmella S.G., Kuo C.T., Siddiqui J., Chen J.H., Oshima K., Huang Y.W., Hecht S.S, & Stoner G.D. (2014). Chemoprevention of esophageal cancer with black raspberries, their component anthocyanins, and a major anthocyanin metabolite, protocatechuic acid. Cancer prevention research (Philadelphia, Pa.), 7(6), 574-584.
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9

Isolation and Cryopreservation of PBMCs

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Peripheral blood was collected in heparin-coated tubes (BD Biosciences, Franklin Lakes, NJ) and separated into plasma and peripheral blood mononuclear cells (PBMCs). Both plasma and PBMCs were stored at −80 °C until use. PBMC and plasma samples from 59 age-, sex-, and race-matched healthy volunteers were included as HC.
Li W., Lin E.L., Liangpunsakul S., Lan J., Chalasani S., Rane S., Puri P., Kamath P.S., Sanyal A.J., Shah V.H., Radaeva S., Crabb D.W., Chalasani N, & Yu Q. (2019). Alcohol Abstinence Does Not Fully Reverse Abnormalities of Mucosal-Associated Invariant T Cells in the Blood of Patients With Alcoholic Hepatitis. Clinical and Translational Gastroenterology, 10(6), e00052.
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10

Jejunum Specimens from Gastric Bypass Surgery

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Jejunum specimens were collected during laparoscopic Roux-En-Y gastric bypass surgery in Departments of Gastroenterology and Surgery, Lithuanian University of Health Sciences (Kaunas, Lithuania). Two cohorts of jejunum surgical samples from unrelated individuals were collected: cohort 1 (n = 56 individuals, 21–72 years old) and cohort 2 (n = 59 individuals, 22–65 years old) (Supplementary Fig. 1a). Following removal, jejunum samples were immediately submerged in RNAlater (Life Technologies), kept overnight at 4°C, followed by storage at −80°C. Blood (n = 58 individuals) and sperm (n = 18 individuals) samples were collected from unrelated individuals enrolled in laparoscopic surgery, and were from separate individuals than those in cohort 1 and 2. Blood samples were collected by venipuncture into heparin-coated tubes (BD), and then stored immediately at −80°C. Sperm samples provided by participants were stored immediately at −80°C. The study protocol was approved by the Bioethics Committee of Lithuanian University of Health Sciences (Protocol 2007-12-04 Nr.BE-2-55), and each patient signed informed consent to participate in the study. Human tissues were processed for mRNA analysis, DNA modification profiling and genotyping by researchers blind to any sample information.
Labrie V., Buske O.J., Oh E., Jeremian R., Ptak C., Gasiūnas G., Maleckas A., Petereit R., Žvirbliene A., Adamonis K., Kriukienė E., Koncevičius K., Gordevičius J., Nair A., Zhang A., Ebrahimi S., Oh G., Šikšnys V., Kupčinskas L., Brudno M, & Petronis A. (2016). Lactase non-persistence is directed by DNA variation-dependent epigenetic aging. Nature structural & molecular biology, 23(6), 566-573.
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