Small animal stereotaxic instrument

Manufactured by Kopf Instruments

The Small-animal stereotaxic instrument is a precision device used to immobilize small animals, such as rodents, during neuroscientific procedures. It allows for the accurate positioning and stabilization of the animal's head, enabling researchers to conduct targeted manipulations or measurements within the brain.

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Lab products found in correlation

Microinfusion pump, by Harvard Apparatus (1 mentions) Icr scid mice, by Taconic Biosciences (1 mentions) C57bl 6 mice, by Charles River Laboratories (1 mentions)

Close spelling variants of this product

Stereotaxic instrument (35 citations) Model 900 Small Animal Stereotaxic Instrument (4 citations) Digital small animal stereotaxic instrument (3 citations)

3 protocols using small animal stereotaxic instrument

Small-Animal Stereotaxic Microinfusion

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Animals were anesthetized and positioned in a small-animal stereotaxic instrument (David Kopf Instruments, Tujunga, CA). A Hamilton syringe needle (33-gauge) was used to unilaterally infuse Lumafluor, virus, or chemicals in a volume of 0.15–0.5 µL at a rate of 0.1 µL/min with a microinfusion pump (Harvard Apparatus, Holliston, MA).

Zhang H., Qian Y.L., Li C., Liu D., Wang L., Wang X.Y., Liu M.J., Liu H., Zhang S., Guo X.Y., Yang J.X., Ding H.L., Koo J.W., Mouzon E., Deisseroth K., Nestler E.J., Zachariou V., Han M.H, & Cao J.L. (2017). Brain-derived neurotrophic factor in the mesolimbic reward circuitry mediates nociception in chronic neuropathic pain. Biological psychiatry, 82(8), 608-618.

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Viral Manipulation and Social Stress in Mice

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Mice were anaesthetized with ketamine (100mg/kg) and xylazine
(10mg/kg) and placed in a small-animal stereotaxic instrument (Kopf
Instruments). HSV virus (0.5μL of either HSV-Sdk1 or HSV-GFP) was
bilaterally infused using 33-gauge syringe needles (Hamilton) into the vHIP
(bregma coordinates: anterior/posterior, −3.7 mm;
medial/lateral, 3 mm; dorsal/ventral, −4.8 mm;
0° angle; targeting ventral subiculum). Two days after viral
infusion, the animals underwent an accelerated defeat protocol in which they
were subjected to social defeat stress twice daily for 10 min over four
days.

Hultman R., Ulrich K., Sachs B.D., Blount C., Carlson D.E., Ndubuizu N., Bagot R.C., Parise E., Vu M.A., Gallagher N.M., Wang J., Silva A.J., Deisseroth K., Mague S.D., Caron M.G., Nestler E.J., Carin L, & Dzirasa K. (2018). Brain-wide electrical spatiotemporal dynamics encode depression vulnerability. Cell, 173(1), 166-180.e14.

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Intracranial Glioma Cell Implantation in Mice

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All animal procedures were approved by the Institutional Animal Care Use Committee of Icahn School of Medicine at Mount Sinai. For implantation of glioma cells into the right striatum of mice, 6- to 8-week-old C57BL/6 mice (Charles River) were used for GL261 implantation and ICR-SCID mice (Taconic) for SD3 GSC implantation. Mice were anesthetized with isoflurane and restrained in a small-animal stereotaxic instrument (David Kopf Instruments). A scalp incision was made, followed by a small cranial opening with a 26-gauge needle tip, 2 mm right and 0.5 mm anterior of Bregma. A Hamilton syringe attached to the stereotaxic frame fitted with a 26-gauge removable needle was used to inoculate glioma cells 2 mm below the cortical surface. The cranial opening was sealed with bone wax to prevent the backflow of cells. The skin was then reapproximated by the placement of interrupted 4-0 Vicryl sutures.

Bozec D., Sattiraju A., Bouras A., Jesu Raj J.G., Rivera D., Huang Y., Junqueira Alves C., Tejero R., Tsankova N.M., Zou H., Hadjipanayis C, & Friedel R.H. (2020). Akaluc bioluminescence offers superior sensitivity to track in vivo glioma expansion. Neuro-oncology Advances, 2(1), vdaa134.

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