Rat brains were cut into 25-μm sections and used for double immunostaining analysis (Han et al., 2016 (link)). Brain sections were incubated with primary antibody and then with the appropriate Alexa 488- or 594-conjugated secondary antibody (Molecular Probes, Eugene, OR). We used the following primary antibodies: rabbit anti-A2bR (1:200, Santa Cruz Biotechnology, Dallas, TX), mouse anti-GFAP (1:500, Sigma-Aldrich, St. Louis, MO), mouse anti-NeuN (1:500, Millipore, Billerica, MA), mouse anti- von Willebrand factor (vWF; 1:50, Dako, Carpinteria, CA), and sheep anti-albumin (1:1000, Bethyl Laboratories, Montgomery, TX). Negative controls were treated with a similar procedure, but the primary antibody was omitted (Lan et al., 2017 (link)). The coverslips were mounted on glass slides and stained with 4′, 6-diamidino-2-phenylindole. The sections were examined under a fluorescence microscope (Eclipse 90i; Nikon, Tokyo, Japan).
Mouse anti von willebrand factor vwf
Manufactured by Agilent Technologies
The mouse anti-von Willebrand factor (vWF) is a laboratory reagent used to detect and measure the presence of von Willebrand factor in biological samples. Von Willebrand factor is a large multimeric glycoprotein involved in platelet adhesion and the coagulation process.
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Lab products found in correlation
Mouse anti gfap, by Merck Group (1 mentions)
Sheep anti albumin, by Fortis Life Sciences (1 mentions)
Eclipse 90i, by Nikon (1 mentions)
Mouse anti neun, by Merck Group (1 mentions)
Mle 12 cells, by American Type Culture Collection (1 mentions)
Exo flow exosome purification kit, by System Biosciences (1 mentions)
Total exosome isolation kit, by Thermo Fisher Scientific (1 mentions)
Exoglow vivo ev labeling kit, by System Biosciences (1 mentions)
Sprague dawley rat, by Charles River Laboratories (1 mentions)
Rabbit anti ki67, by Abcam (1 mentions)
2 protocols using mouse anti von willebrand factor vwf
1
Immunostaining Analysis of Rat Brain Sections
2
Isolation and Characterization of Extracellular Vesicles from Pregnant Rat Lungs
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Pregnant Sprague-Dawley rats were purchased from Charles River Laboratory (Wilmington, MA). The following antibodies were used for immunostaining, immunofluorescence staining, Western blot analysis and FACS analysis: rabbit anti-GSDMD and mouse anti-AIF-1 from Novusbio (Littleton, CO); rabbit anti-pro-SPC and mouse anti-CD63 from EDMillipore (Temecula, CA); mouse anti-CD9 antibody from ThermoFisher (Waltham, MA); rabbit anti-Ki67 from Abcam (Cambridge, MA); mouse anti-vonWillebrand factor (vWF) from Dako (Carpinteria, CA); rabbit anti-SPC-PE from Biossusa (Woburn, MA). Total Exosome Isolation Kit was obtained from ThermoFisher. Exo-FLOW Exosome Purification Kit and ExoGlow-Vivo EV Labeling Kit were obtained from Systems Biosciences (Palo Alto, CA). The lipophilic fluorescent dye, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarocyanine perchlorate (DiI) was purchased from Sigma (Louis, MO). MLE-12 cells were obtained from ATCC (Manassas, VA), pulmonary vascular endothelial cells (PVEC) were obtained from Lonza (Walkersville, MD), and rat fetal neural stem cells (NSC) were obtained from ThermoFisher. The primers for all the qRT-PCR were obtained from ThermoFisher.
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