Agarose

All metal chlorides, formamide, and CAPS buffer were purchased from Sigma-Aldrich. Tris base was purchased from J. T. Baker and glacial acetic acid was from Mallinckrodt Chemicals. Ethylenediaminetetraacetic acid (EDTA)-disodium salt was obtained from EMD Chemicals, Inc. The 2-Log DNA ladder, also called 1 kb Plus DNA ladder, 1 kb Extend DNA ladder, bacteriophage lambda DNA and M13mp18 DNAs were purchased from New England Biolabs. E. coli chromosomal DNA (D2001) was obtained from Sigma-Aldrich. Agarose was from Gold Biotechnology.

Powdered sodium montmorillonite (Na-MMT) was obtained from Southern Clay Products, Inc. This form of MMT is Na⁺ Cloisite, which is a water-washed homoionic sodium form of the clay having a cation exchange capacity of 95 meq/100 g. Purified E. coli transfer RNA(tRNA), tricine, triethanolamine, magnesium chloride, sodium chloride, sodium carbonate, and sodium sulfate were purchased from Sigma-Aldrich. Agarose was obtained from Gold Biotechnology. Boric acid and Tris base were from JT Baker. SYBR Gold was manufactured by Invitrogen Life Sciences. Ethidium bromide was from IBI Scientific. Eppendorf Flex-Tubes (1.5 mL) were purchased from VWR. Electrophoresis was performed using 11 cm × 14 cm Horizon gel rigs (Labrepco) and an EPS 601 power supply (GE Healthcare).
Oligonucleotides were purchased from Integrated DNA Technologies or from Invitrogen/ThermoFisher Scientific. Sequences of the oligomers were as follows:
PvuRNA (AAAUGAGUCACCCAGAUCUAAAUAA), cPvuRNA (UUAUUUAGAUCUGGGUGACUCAUUU), Pvu4a (AAATGAGTCACCCAGATCTAAATAA), cPvu4a (TTATTTAGATCTGGGTGACTCATTT), dsRNA 54mer RNALoop (AAAUGAGUCACCCAGAUCUAAAUAAGUAAUUAUUUUAGAUCUGGGUGACUCAUU), and ssRNA 54mer RNAStr8 (AAAUGAGUCACCCAGAUCUAAAUAAGUAAAAAUGAGUCACCCAGAUCUAAAUAA).