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Rabbit ant chicken

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Rabbit anti-chicken is a laboratory reagent used to detect and quantify the presence of chicken proteins in biological samples. It is a polyclonal antibody produced by immunizing rabbits with chicken proteins. This reagent can be used in various immunoassay techniques, such as ELISA, Western blot, and immunohistochemistry, to identify and measure chicken-derived molecules in research and diagnostic applications.

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Lab products found in correlation

Primary antibody against type 1 collagen, by Abcam (2 mentions) Total h3, by Abcam (2 mentions) Pvdf membrane, by Thermo Fisher Scientific (2 mentions) Chemidoc touch imaging system, by Bio-Rad (2 mentions) Gapdh, by Merck Group (2 mentions) Clarity western ecl substrate, by Bio-Rad (2 mentions) Bis tris gel, by Thermo Fisher Scientific (2 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) H3k27me3, by Cell Signaling Technology (2 mentions)

2 protocols using rabbit ant chicken

1

Immunoblotting of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed on tissues that were lysed using ice-cold RIPA buffer supplemented with Halt Phosphatase and Protease Inhibitor Cocktail (Thermo Fisher Scientific). The samples were spun at 3,000g to remove debris and 10 μg of sample was resolved on a 4–12% Bis-Tris gel (Thermo Fisher) using SDS-PAGE. Samples were transferred onto a PVDF membrane (Thermo Fisher) and blocked in 5% milk in PBS with 0.1% Tween 20 (PBS-T). Membranes were washed 3 times in PBS-T after blocking and between each subsequent step. Membranes were incubated overnight at 4°C with primary antibody against type I collagen (Abcam, 1:1000), GAPDH (EMD Millipore, 1:1000), H3K27me3 (Cell Signaling Technology, 1:000), or total H3 (Abcam, 1:000) diluted in 1% Bovine Serum Albumin in PBS-T with 0.05% sodium azide. Subsequently, membranes were incubated with species-specific HRP-conjugated antibodies (goat anti-rabbit (Abcam); rabbit ant-chicken (EMD Millipore)), developed with Clarity Western ECL Substrate (Bio-Rad), and visualized with a ChemiDoc Touch Imaging System (Bio-Rad).
Osokine I., Siewiera J., Rideaux D., Ma S., Tsukui T, & Erlebacher A. (2022). Gene silencing by EZH2 suppresses TGF-β activity within the decidua to avert pregnancy-adverse wound healing at the maternal-fetal interface. Cell reports, 38(5), 110329.
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2

Immunoblotting of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed on tissues that were lysed using ice-cold RIPA buffer supplemented with Halt Phosphatase and Protease Inhibitor Cocktail (Thermo Fisher Scientific). The samples were spun at 3,000g to remove debris and 10 μg of sample was resolved on a 4–12% Bis-Tris gel (Thermo Fisher) using SDS-PAGE. Samples were transferred onto a PVDF membrane (Thermo Fisher) and blocked in 5% milk in PBS with 0.1% Tween 20 (PBS-T). Membranes were washed 3 times in PBS-T after blocking and between each subsequent step. Membranes were incubated overnight at 4°C with primary antibody against type I collagen (Abcam, 1:1000), GAPDH (EMD Millipore, 1:1000), H3K27me3 (Cell Signaling Technology, 1:000), or total H3 (Abcam, 1:000) diluted in 1% Bovine Serum Albumin in PBS-T with 0.05% sodium azide. Subsequently, membranes were incubated with species-specific HRP-conjugated antibodies (goat anti-rabbit (Abcam); rabbit ant-chicken (EMD Millipore)), developed with Clarity Western ECL Substrate (Bio-Rad), and visualized with a ChemiDoc Touch Imaging System (Bio-Rad).
Osokine I., Siewiera J., Rideaux D., Ma S., Tsukui T, & Erlebacher A. (2022). Gene silencing by EZH2 suppresses TGF-β activity within the decidua to avert pregnancy-adverse wound healing at the maternal-fetal interface. Cell reports, 38(5), 110329.
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