Anti hec1 ab3613

Manufactured by Abcam

Sourced in United States

Anti-HEC1 (ab3613) is a primary antibody that recognizes the HEC1 protein. HEC1 is a component of the kinetochore complex and plays a critical role in chromosome segregation during cell division. This antibody can be used in various applications, such as Western blotting and immunofluorescence, to detect and study the HEC1 protein.

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Lab products found in correlation

Anti bubr1, by BD (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Axiovert 200m microscope, by Zeiss (1 mentions) Anti aurora b, by BD (1 mentions) Anti cyclin b1, by Cell Signaling Technology (1 mentions) Mouse anti his tag, by Cell Signaling Technology (1 mentions) Mouse anti tubulin, by Cell Signaling Technology (1 mentions) Rabbit anti gfp, by Proteintech (1 mentions) Mouse anti flag, by Merck Group (1 mentions)

Close spelling variants of this product

Ab3613 (8 citations) Anti-HEC1 (2 citations)

2 protocols using anti hec1 ab3613

Immunofluorescence Visualization of Mitotic Proteins

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Immunofluorescence was performed as described previously [30 (link)] using the following antibodies: anti-CENP-B (sc22788; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-HEC1 (ab3613; Abcam, Cambridge, UK), anti-BubR1 (612503; BD Transduction Laboratories, San Jose, CA, USA), and anti-α-tubulin (T9026; Sigma-Aldrich). Images were captured with a Plan-APOCHROMAT 100× oil lens on an Axiovert 200M microscope (Carl Zeiss, Jena, Germany).

Ohashi A., Ohori M., Iwai K., Nambu T., Miyamoto M., Kawamoto T, & Okaniwa M. (2015). A Novel Time-Dependent CENP-E Inhibitor with Potent Antitumor Activity. PLoS ONE, 10(12), e0144675.

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Antibody Characterization and Detection

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Rabbit anti-CENP-R (10743-1-AP, Proteintech), mouse anti-tubulin (3873, Cell Signaling Technology), rabbit anti-GFP (50430-2-AP, Proteintech), mouse anti-His tag (23665, Cell Signaling Technology), mouse anti-FLAG (F3165, Sigma), anti-Cyclin B1 (12231, Cell Signaling Technology), anti-Hec1 (AB3613, Abcam), anti-Aurora B (611082, BD), anti-CENP-U (HPA022048, Atlas), and human anti-centromere auto-antibody (ACA, HCT-0100, Immunovision) were obtained commercially. For all western blotting, signals were detected using HRP-conjugated anti-mouse or anti-rabbit antibodies (Pierce).

Sedzro D.M., Yuan X., Mullen M., Ejaz U., Yang T., Liu X., Song X., Tang Y.C., Pan W., Zou P., Gao X., Wang D., Wang Z., Dou Z., Liu X, & Yao X. (2022). Phosphorylation of CENP-R by Aurora B regulates kinetochore–microtubule attachment for accurate chromosome segregation. Journal of Molecular Cell Biology, 14(7), mjac051.

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