Chemokines were measured in mouse serum or skin protein extracts (n=10–12 mice/treatment/sample type) with a Bio-Plex Array Reader (LUMINEX 100; Bio-Rad Laboratories, Hercules, CA) using Milliplex panels (EMD Millipore, Billerica, MA): CCL2/monocyte chemoattractant protein 1, CCL3/macrophage inflammatory protein 1α and CCL5/RANTES. Protein extracts were obtained from skin specimens harvested after one week of treatment, weighed, and frozen in liquid nitrogen. Each sample was homogenized with a mortar and pestle and digested for 2 h at 4°C in Reporter lysis buffer (Promega, Madison, WI) supplemented with complete mini-protease inhibitor tablets (Roche Diagnostics, Indianapolis, IN). Supernatants were stored at −80°C until use.
Bio plex array reader luminex 100
Manufactured by Bio-Rad
Sourced in United States
The Bio-Plex Array Reader (LUMINEX 100) is a multiplex assay system that utilizes flow cytometry technology to analyze multiple analytes simultaneously in a single sample. The instrument is designed to detect and quantify a variety of targets, including proteins, nucleic acids, and other biomolecules.
Automatically generated - may contain errors
3 protocols using bio plex array reader luminex 100
1
Quantification of Chemokines in Mouse Samples
2
Quantifying Chemokine Levels in Mouse Skin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCL2, CCL3 and CCL5 chemokine quantifications were conducted in mouse serum and whole skin protein extracts prepared exactly as previously described [18 (link)], using a Bio-Plex Array Reader (LUMINEX 100; Bio-Rad Laboratories, Hercules, CA, USA) and Milliplex panels (EMD Millipore, Billerica, MA, USA). Whole skin protein extracts were weighed and immediately snap-frozen using liquid nitrogen. After homogenization with a mortar and pestle, skin samples were digested for 2 hours at 4 °C in reporter lysis buffer (Promega, Madison, WI, USA) supplemented with complete mini-protease inhibitors (Roche Diagnostics, Indianapolis, IN, USA). Supernatants were kept at −80 °C until use.
3
Quantifying Chemokine Levels in Mouse Skin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CCL2, CCL3 and CCL5 chemokine quantifications were conducted in mouse serum and whole skin protein extracts prepared exactly as previously described [18 (link)], using a Bio-Plex Array Reader (LUMINEX 100; Bio-Rad Laboratories, Hercules, CA, USA) and Milliplex panels (EMD Millipore, Billerica, MA, USA). Whole skin protein extracts were weighed and immediately snap-frozen using liquid nitrogen. After homogenization with a mortar and pestle, skin samples were digested for 2 hours at 4 °C in reporter lysis buffer (Promega, Madison, WI, USA) supplemented with complete mini-protease inhibitors (Roche Diagnostics, Indianapolis, IN, USA). Supernatants were kept at −80 °C until use.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!