U2OS cells were treated with either JQ1 or DMSO vehicle for 18 h and harvested for either immunoblots or ChIP analysis. For ChIP-qPCR, cells (3×106) were cross-linked with 1% formaldehyde for 15 min at room temperature and sheared (1% SDS, 45 minutes, 100% amplitude) in a sonicator (Qsonica) to produce ~300 bp fragments. Lysates were subjected to immunoprecipitation with Dynabeads Protein G-coupled magnetic beads (Life Technologies) after retaining 10% as input. Recovered DNA was subjected to qPCR with locus specific primer pairs. The results were normalized to the input DNA.
Dynabeads protein g coupled magnetic beads
Manufactured by Thermo Fisher Scientific
Dynabeads Protein G-coupled magnetic beads are a type of lab equipment used for immunoprecipitation and affinity purification. These beads are made of polystyrene and coated with Protein G, a bacterial protein that binds to the Fc region of antibodies. The magnetic properties of the beads allow them to be easily separated from the reaction mixture using a magnet.
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Lab products found in correlation
2 protocols using dynabeads protein g coupled magnetic beads
1
Chromatin Immunoprecipitation of JQ1 Targets
2
Chromatin Immunoprecipitation of JQ1 Targets
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