The protein expression was assessed by Western blotting. Primary antibodies, i.e., mouse anti-α-SMA antibody (SMA, Sigma-Aldrich, A2547), rabbit anti- MMP-9 antibody (Abcam, Cambridge, UK, ab76003), mouse anti-Toll-like receptor-4 (TLR-4) antibody (Abcam, ab30667), and mouse anti-β-actin antibody for internal control (Sigma-Aldrich, A5411) were reacted with the polyvinylidene fluoride (PVDF) membrane at 4 °C overnight. Subsequently, the corresponding secondary antibodies were immersed at room temperature for 1 h. The protein bands were quantified using Image J software and normalized using individual internal controls for comparison [17 (link),18 (link)].
Mouse anti
Manufactured by Abcam
Sourced in United Kingdom
Mouse anti is a laboratory reagent used for the detection and identification of target proteins or molecules in biological samples. It functions as a primary antibody that specifically binds to a corresponding antigen, allowing for further analysis or detection of the target of interest.
Automatically generated - may contain errors
2 protocols using mouse anti
1
Protein Expression Analysis by Western Blotting
2
Antibody Validation for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The RNF5 antibody was described previously 7, (link)41 . Other antibodies were obtained as follows: rabbit anti-cleaved caspase 3 (#9661, Cell Signaling Technology), rabbit anti-PARP (#9532, Cell Signaling Technology), mouse anti-RBBP4 (NBP1-41201, Novus Biologicals), mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH; ab8245, Abcam), mouse anti-
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