Plasmid pet 30a

Plasmid pET-30a (+) and E. coli BL21 (DE3) were purchased from Tiangen Biotech Co., Ltd. (Beijing, China). Soluble starch, agarose, carrageen, sodium cellulose, and alginate were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, china). Grateloupia filicina, Chondrus ocellatus, and Scagassum were collected from Yangkou Beach in Qingdao, China.

Plasmid pET-30a (+) and E. coli BL21 (DE3) were purchased from Tiangen (Beijing, China). Agarose was purchased from Sinopharm Group Chemical Reagent (Shanghai, China). His-tag-specific purification kits were purchased from GE Healthcare (Pittsburgh, PA, United States). Thin layer chromatography (TLC) silica gel plates were purchased from Kadel (Shanghai, China). Bio-Gel P-2 was purchased from Bio-Rad (Pittsburgh, PA, United States). RAW 264.7 macrophages were obtained from the Cell Bank, Chinese Academy of Sciences (Shanghai, China). LPS and L-monomethylarginine (L-NMMA) were purchased from Yuanye (Shanghai, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and dimethyl sulfoxide (DMSO) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Fetal bovine serum (FBS) was obtained from Gibco (Grand Island, NY, United States). Enzyme Linked Immunoabsorbent Assay (ELISA) kits (TNF-α and IL-6) were purchased from AndHider (Qingdao, Shandong, China).