The chemicals used were analytical reagent grade: ABTZ (ABTZ, Sigma-Aldrich USA), DPPH (DPPH, Sigma-Aldrich USA), Trolox (Sigma-Aldrich USA), Folin-Ciocalteu phenol reagent (Sigma-Aldrich USA), Sodium carbonate (Na 2 CO 3 , Univar Ajax Finechem New Zealand) Aluminium chloride (AlCl 3 , Univar Ajax Finechem New Zealand), Potassium acetate (CH 3 COOK; Univar Ajax Finechem New Zealand), Hydrochloric acid (Lab Scan Thailand), Ethanol (Lab Scan Thailand). HPLC grade: 4,5-O-dicaffeoylquinic acid (Sigma-Aldrich USA), Quercetin (Sigma-Aldrich USA), Gallic acid (Sigma-Aldrich USA), MEthanol (Lab Scan Thailand), Phosphoric acid (Lab Scan Thailand), and Cosmetic grade: Tween 80 and propylene glycol (Namsiang Thailand).
Phosphoric acid
Manufactured by RCI Labscan
Sourced in Thailand
Phosphoric acid is a colorless, odorless, and highly corrosive inorganic compound. It is a strong acid with a chemical formula of H3PO4. Phosphoric acid is primarily used in industrial applications as a reagent or a chemical intermediate.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Merck Group (3 mentions)
Quercetin, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by RCI Labscan (2 mentions)
Methanol, by RCI Labscan (2 mentions)
Ethanol, by Merck Group (2 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Folin ciocalteu phenol reagent, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
4 5 o dicaffeoylquinic acid, by Merck Group (1 mentions)
Ethanol, by RCI Labscan (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Ammonia solution, by Merck Group (1 mentions)
Sodium borohydride, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by RCI Labscan (1 mentions)
Tetraethyl orthosilicate, by Merck Group (1 mentions)
Sodium hydrogen carbonate, by Merck Group (1 mentions)
Tellurium powder, by Merck Group (1 mentions)
Thioglycolic acid, by Merck Group (1 mentions)
Salbutamol, by Merck Group (1 mentions)
8 protocols using phosphoric acid
1
Phytochemical Screening of Botanical Extracts
2
Synthesis of Tellurium-Cadmium Nanoparticles
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Tellurium powder (-200 mesh, 99.8%), cadmium chloride (CdCl2.2H2O), sodium borohydride (NaBH4), 3-aminopropyltriethoxysilane (APTES), tetraethyl orthosilicate (TEOS), thioglycolic acid (TGA) and salbutamol were purchased from Sigma-Aldrich (St.
Louis, MO, USA). Sodium carbonate, sodium hydrogen carbonate, ammonia solution, acetonitrile, methanol and ethanol were obtained from Merck (Darmstadt, Germany).
Phosphoric acid and sodium hydroxide were from Labscan (Bangkok, Thailand). Deionized water was obtained from a Maxima ultrapure water system (18.2 M) (Elgastat Maxima, ELGA, UK).
Louis, MO, USA). Sodium carbonate, sodium hydrogen carbonate, ammonia solution, acetonitrile, methanol and ethanol were obtained from Merck (Darmstadt, Germany).
Phosphoric acid and sodium hydroxide were from Labscan (Bangkok, Thailand). Deionized water was obtained from a Maxima ultrapure water system (18.2 M) (Elgastat Maxima, ELGA, UK).
3
Antioxidant and Anti-inflammatory Assays
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Brazilin, ellagic acid, eugenol, myristicin, piperine, gallic acid, and propyl gallate (purity > 98%) were purchased from Sigma-Aldrich (MO, USA). Folin-Ciocalteus’s phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and butylated hydroxytoluene (BHT) were purchased from Fluka (Munich, Germany). Dimethyl sulfoxide (DMSO), acetonitrile, and phosphoric acid were purchased from RCI Lab Scan (Bangkok, Thailand). Purified water was prepared by Milli Q®system from Millipore (Bedford, MA, USA).
Murine macrophage leukemia cell line (RAW 264.7: ATCC® TIB-71™) and Human promyelocytic leukemia cells (HL-60) were purchased from American Type Culture Collection (VA, USA). Roswell Park Memorial Institute medium 1640 (RPMI-1640), fetal bovine serum (FBS), penicillin-streptomycin, phosphate buffer saline (PBS), and trypsin-EDTA were purchased from Biochrom (MA, Germany). Lipopolysaccharide (LPS), 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium bromide (MTT) and nitroblue tetrazolium (NBT) were purchased from Sigma-Aldrich. (MO, USA).
Murine macrophage leukemia cell line (RAW 264.7: ATCC® TIB-71™) and Human promyelocytic leukemia cells (HL-60) were purchased from American Type Culture Collection (VA, USA). Roswell Park Memorial Institute medium 1640 (RPMI-1640), fetal bovine serum (FBS), penicillin-streptomycin, phosphate buffer saline (PBS), and trypsin-EDTA were purchased from Biochrom (MA, Germany). Lipopolysaccharide (LPS), 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium bromide (MTT) and nitroblue tetrazolium (NBT) were purchased from Sigma-Aldrich. (MO, USA).
4
Antioxidant Evaluation of P. indica Extract
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Branches of P. indica were collected from the herbal garden of the Faculty of Pharmacy, Srinakharinwirot University, in August 2018 and was identified by Assistant Professor Dr. Sirivan Athikomkulchai. The plant specimen was deposited (SIRA002) at the herbarium of the Faculty of Pharmacy, Srinakharinwirot University. Primary Epidermal keratinocytes (ATCC® Number: PCS-200-011), human dermal fibroblast cells line (HDFB) and dermal cell basal medium plus keratinocyte growth kit were purchased from the American Type Culture Collection (Manassas, VA, USA). Dulbecco’s modified eagle’s medium (DMEM), fetal bovine serum (FBS), trypsin-EDTA, sodium carboxymethyl cellulose, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), Epigallocatechin gallate (EGCG) standard, gallic acid standard, sodium nitrite, and sodium hydroxide, and dimethyl sulfoxide (cell culture grade) were purchased from Sigma Aldrich (St. Louis, MO, USA). Folin-Ciocaleu reagent, 95% ethanol, sodium carbonate, and aluminium chloride were purchased from Merck & Co., Inc. (Kenilworth, NJ, USA). Penicillin-streptomycin and phosphate-buffered saline were purchased from Gibco Thermo Fisher Scientific (Waltham, MA, USA). Phosphoric acid and acetonitrile were purchased from RCI Labscan, Bangkok, Thailand.
5
Comprehensive Chemical Analysis Protocol
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All of the chemicals used
were of analytical grade. Citric acid, mercury chloride, potassium
cyanide, and boric acid were obtained from Qrec (New Zealand). Potassium
ferricyanide was purchased from BOH Chemicals Ltd. (England). Sodium
hydroxide was obtained from Carlo Erba (Italy). Phosphoric acid and
acetic acid were purchased from RCI Labscan Limited (Thailand). Ethylene
diaminetetraacetic acid was purchased from UNIVAR (Australia). Paraffin
oil was purchased from Ajex Finechem (Australia). Deionized water
(Simplicity Water Purification System, Model Simplicity 185, Millipore,
U.S.A.) was used for all the experiments.
were of analytical grade. Citric acid, mercury chloride, potassium
cyanide, and boric acid were obtained from Qrec (New Zealand). Potassium
ferricyanide was purchased from BOH Chemicals Ltd. (England). Sodium
hydroxide was obtained from Carlo Erba (Italy). Phosphoric acid and
acetic acid were purchased from RCI Labscan Limited (Thailand). Ethylene
diaminetetraacetic acid was purchased from UNIVAR (Australia). Paraffin
oil was purchased from Ajex Finechem (Australia). Deionized water
(Simplicity Water Purification System, Model Simplicity 185, Millipore,
U.S.A.) was used for all the experiments.
6
Sugarcane Filter Cake Valorization
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Dried sugarcane filter cake was obtained from Mitr Phol sugar factory (Chaiyaphum province, Thailand). The as-received filter cake was milled by ball mill to obtain a homogeneous powder. Phosphoric acid (H3PO4, ≥85%, RCI Labscan, Thailand), magnesium oxide (MgO, ≥97%, RCI Labscan, Thailand), calcium oxide (CaO, ≥90%, Kemaus, Australia), formic acid (99%, APS Chemicals, New Zealand), and ammonium persulfate (98%, Loba Chemie, India) were used as received. All solutions were prepared with DI water (18.2 MΩ).
7
Sourcing Chemicals for Research
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The organic solvents including hexane, ethyl acetate, and methanol were purchased from S.C. Science (Thailand). Phosphoric acid and hydrochloric acid (HCl) were obtained from RCI labscan (Thailand). Dimethyl sulfoxide (DMSO) was supplied from Amresco Inc. (USA). 2,4,6-tripyridyl-s-triazine (TPTZ), 2,2-diphenyl-1picrylhydrazyl (DPPH) and hydrocortisone solution were obtained from Sigma-Aldrich (Missouri, USA). Iron (III) chloride hexahydrate (FeCl 3 •6H 2 O) was purchased from AnalaR (England). Dulbecco's Modified Eagle Medium (DMEM), Ham F'12 and bovine serum albumin (BSA) were obtained from Gibco BRL (New York, USA).
8
Vegetable Extract Preparation and Analysis
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The organic solvents used for vegetable extraction, including hexane, ethyl acetate, and methanol were purchased from S.C. Science Co., Ltd. (Thailand). methanol and phosphoric acid, HPLC grade, were purchased from RCI labscan (Thailand). Dimethyl sulfoxide (DMSO) was obtained from Amresco Inc. (USA). Catechin, quercetin, ferulic acid, rutin, caffeine, gallic acid and neutral red were obtained from Sigma-Aldrich Co. LLC (USA). Dulbecco's Modified Eagle's Medium (DMEM), bovine serum albumin (BSA), penicillin-streptomycin and trypsin-EDTA were obtained from Gibco BRL (Grand Island, NY, USA).
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