The dental cell re-associations were cultured for 7 days and further co-cultured overnight with trigeminal ganglia (TG) before implantation between skin and muscles, behind the ears of ICR (152 samples) (Charles River Laboratories, l′Arbresle, France), CsA-treated ICR (229 samples) or Nude (NMRI-nu/nu, Janvier Labs, Saint Berthevin, France) (98 samples) adult mice [2] (link). Cell re-associations were also implanted without trigeminal ganglia and used as controls (42 samples in ICR, 55 samples in CsA-treated ICR and 28 samples in Nude adult mice). The mice were anaesthetized by intraperitoneal injection of 100 mg/g of ketamine (Virbac, Centravet, Nancy, France) and 10 mg/g of Xylazine (Rompun® 2%, Centravet, Nancy, France). The implantations were maintained in vivo for one or two weeks. Then, implanted mice were sacrificed by lethal injection of pentobarbital (Centravet, Nancy, France) and the implants were harvested for either histological analysis, or immunostaining, or transmission electron microscopy (TEM).
Nmri nu nu
Manufactured by Janvier Labs
Sourced in France
The NMRI nu/nu is a laboratory equipment designed for nuclear magnetic resonance imaging (NMRI) applications. It is a specialized device used to conduct imaging and analysis of biological samples. The core function of this equipment is to generate and detect magnetic resonance signals, which can be used to obtain detailed information about the structure and composition of the samples under investigation.
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Lab products found in correlation
5 protocols using nmri nu nu
1
Dental cell re-associations and trigeminal ganglia implantation
2
Preclinical Evaluation of PI3K and EGFR Inhibitors
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The pan-class I PI3K inhibitor copanlisib (BAY 841236) was manufactured by Bayer AG (Germany), and the EGFR inhibitor cetuximab was obtained from Merck (Darmstadt, Germany). The vehicle used was 5% mannitol in water for copanlisib and saline (0.9% NaCl) for cetuximab [18 (link)].
The PDX models were established at EPO Berlin-Buch GmbH (Germany) and propagated subcutaneously in NMRI nu/nu or NOG (for HN15239) mice (Janvier, France) as described previously [18 (link)]. EPO is fully accredited by AAALAC. For transplantation, tumors were harvested and cut into small fragments. The study was approved by the local Institutional Review Board of Charité University Medicine, Germany (EA4/019/12). All animal experiments were carried out in accordance with the United Kingdom coordinating committee on cancer research regulations for the welfare of animals and the German Animal Protection Law and were also approved by the local responsible authorities (LaGeSoBerlin, A0452/08).
The PDX models were established at EPO Berlin-Buch GmbH (Germany) and propagated subcutaneously in NMRI nu/nu or NOG (for HN15239) mice (Janvier, France) as described previously [18 (link)]. EPO is fully accredited by AAALAC. For transplantation, tumors were harvested and cut into small fragments. The study was approved by the local Institutional Review Board of Charité University Medicine, Germany (EA4/019/12). All animal experiments were carried out in accordance with the United Kingdom coordinating committee on cancer research regulations for the welfare of animals and the German Animal Protection Law and were also approved by the local responsible authorities (LaGeSoBerlin, A0452/08).
3
Xenograft Tumor Growth Inhibition
4
In Vivo Multispectral Imaging of Atherosclerotic Plaques
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Three nude mice (NMRI nu/nu, Janvier, France) were imaged in the MSOT system. Bio-Gel Polyacrylamide Gel (Bio-Gel) (Bio-Rad, Hercules, CA, USA) was prepared and mixed with 200 μM Nc-Cremophor mix to a final concentration of 5 μM. IRDye was also mixed with Bio-Gel to a final concentration of 5 μM. 100 μL of each Bio-Gel/dye mixture was injected subcutaneously on the back of each mouse [91] . The mice were placed in the MSOT and imaged tomographically in the region of the plaques with excitation wavelengths from 680 to 900 nm at every 5 nm. Images were analyzed using MEDgical. Volumes-of-interest were drawn around the Bio-Gel pellets and spectra were exported. PA spectra were averaged across 3 mice and were plotted using GraphPad Prism 7.
5
Subcutaneous Implantation of MSC-Seeded Biomaterials
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The in vivo experiment was conducted according to the European regulation (Directive 2010/63/UE). The approval of the study was obtained from the local ethical committee on animal experiment (number6575). Eighteen 7 week-old female nude mice were used (NMRI nu/nu, Janvier Labs). Six groups were considered: BCP; BMMSC + BCP; NE-MSC + BCP; BG; BMMSC + BG; NE-MSC + BG on 50 mg of BCP or 90 mg of BG. No pre-treatment was performed with BG granules. 2 × 106 cells were seeded on biomaterial granules and incubated overnight at 37°C with 5% CO2, prior to implantation. Two identical subcutaneous implantation per mouse were performed. For statistical purpose, six implants per group were considered. Animals were operated under general anaesthesia with Isoflurane (Abbvie, North Chicago, Illinois, United States). A centimetric skin incision was performed on each side of mouse spine. Subcutaneous pockets were filled with granules, embedded with or without MSCs. Wounds were closed with non-resorbable suture 4.0 Filapeau (Péters Surgicals, Bobigny, France). Animals were controlled every day post-surgery, to monitor the healing of the skin and behavioural anomalies. Eight weeks post-implantation, animals were euthanized, and implants were fixed in 4% PFA.
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