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Spectralon srt 99 100

Manufactured by Labsphere
Sourced in United States, United Kingdom

Spectralon SRT-99-100 is a highly reflective, Lambertian white material used as a diffuse reflectance standard. It provides a near-perfect reflection of incident radiation across the ultraviolet, visible, and near-infrared spectrum.

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2 protocols using spectralon srt 99 100

1

Hyperspectral Imaging for Sample Analysis

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The hyperspectral image system mainly includes a hyperspectral camera (SWIR-CL-400-N25E, SPECIM, Finland), two 150-Watt halogen lamps, two focusing lenses, and a mobile sorting platform setup, as show in Figure 2. Before collecting hyperspectral images, the lamp was turned on for about 15 min, and the white and black calibration images of the hyperspectral camera were captured. The white calibration image was acquired from a 99.9% reflectance white board (Spectralon SRT-99-100, Labsphere Inc., North Sutton, NH, USA) 30 cm below the camera. The black calibration image was acquired when the lens was completely shielded.
The hyperspectral images of samples were corrected using Equation (1):
Here, Idata represents the acquired hyperspectral image, Iblack represents the black calibration image, Iwhite represents the white calibration image, and I represents the corrected image. The exposure time and the frame rate of the hyperspectral camera were 1.8 ms and 120 f/s, respectively.
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2

Broadband Diffuse Reflectance Spectroscopy

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DRS measurements were obtained between 400 and 1600 nm. Details on the measurement setup were reported previously.28 (link),29 (link) In short, the light of a halogen light source was transferred to the tissue by means of the illuminating fiber that is integrated into a fiber-optic probe. Two other integrated fibers were attached to two spectrometers, one covering the visual wavelength range (Andor Technology, DU420A-BRDD, 400 to 1000 nm) and one covering the near-infrared wavelength range (Andor Technology, DU492A-1.7, 900 to 1600 nm), both of which collected the photons after interacting with the tissue. The setup was controlled with LabVIEW® (Austin, Texas) software that performed a calibration from photon counts to diffuse reflectance and combined the output of both spectrographs to form one continuous spectrum from 400 to 1600 nm. For the calibration, a cap with Spectralon® (SRT-99-100, Labsphere, Inc., Northern Sutton, New Hampshire) at the bottom was used. The fiber distance between the emitting and collecting fiber was 1 mm.
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