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2 protocols using optima liquid chromatography mass spectrometry lc ms grade

1

Metabolomic Analysis of Cell Lines

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FBS, penicillin, and streptomycin were purchased from HyClone Laboratories. RPMI 1640 medium and charcoal-stripped FBS were purchased from Thermo Fisher. Dialyzed FBS was from Life Technologies. Optima ammonium acetate, ammonium hydroxide, Optima liquid chromatography–mass spectrometry (LC-MS) grade, acetonitrile, methanol, and water were purchased from Fisher Scientific. U-13C glucose, U-13C glutamine, and 2,3,3-2H serine were obtained from Cambridge Isotope Laboratories. U-13C palmitate, C16-ceramide, and C2-ceramide were purchased from Sigma-Aldrich.
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2

Metabolomic Analysis of Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
FBS, penicillin, and streptomycin were purchased from HyClone Laboratories. RPMI 1640 medium and charcoal-stripped FBS were purchased from Thermo Fisher. Dialyzed FBS was from Life Technologies. Optima ammonium acetate, ammonium hydroxide, Optima liquid chromatography–mass spectrometry (LC-MS) grade, acetonitrile, methanol, and water were purchased from Fisher Scientific. U-13C glucose, U-13C glutamine, and 2,3,3-2H serine were obtained from Cambridge Isotope Laboratories. U-13C palmitate, C16-ceramide, and C2-ceramide were purchased from Sigma-Aldrich.
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