The pHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry (ThermoFisher Scientific) was used according to the manufacturer’s instructions. Briefly, cells were incubated with pHrodo™ BioParticles® conjugate for 15 min on either ice (negative control) or 37 degrees Celsius (experimental conditions) and then stained with antibodies followed by flow cytometric analysis.
Phrodo red e coli bioparticles phagocytosis kit for flow cytometry
Manufactured by Thermo Fisher Scientific
Sourced in United States
The PHrodo™ Red E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry is a reagent used to study phagocytosis in cells. It contains fluorescently-labeled E. coli particles that become more fluorescent when internalized by phagocytic cells, allowing for the measurement of phagocytic activity using flow cytometry.
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3 protocols using phrodo red e coli bioparticles phagocytosis kit for flow cytometry
1
Analyzing Phagocytosis via Flow Cytometry
2
Phagocytosis Evaluation by Flow Cytometry
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Phagocytosis was assessed by a well-trained biochemist at the Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University. To evaluate phagocytosis, we used the pHrodo™ Red E. coli BioParticles® Phagocytosis Kit for flow cytometry (Thermo Fisher Scientific Corporation, California, United States). This assay has been validated and used in previous studies (21–23 (link)).
3
Phagocytosis Assay for Macrophages
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Phagocytosis was measured with the pHrodo® Red E. coli BioParticles® Phagocytosis Kit for Flow Cytometry (Thermo Fisher Scientific, Waltham, MA, USA) as per the manufacturer's protocol. Monocyte‐derived macrophages were detached by pipetting following a 30‐min incubation on ice in PBS + 5 mM EDTA and 12 mM lidocaine (both Sigma‐Aldrich).
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