Chlorfenapyr

Azoxystrobin, boscalid, imazalil, chlorfenapyr, isoxathion, and nitenpyram, as shown in Fig. 2 and which were of analytical grade for pesticide residue analysis, were purchased from Fujifilm Wako Pure Chemical Co. (Osaka, Japan). These pesticides were dissolved with methanol (1.0 mg/mL) and kept at 4°C until use for SPR immunosensor or with acetonitrile (1.0 mg/mL) for high performance LC (HPLC) and LC with tandem MS detectors (LC-MS/MS). These pesticides were modified with carboxylic acid as shown in Fig. 2, called haptens in this study, and synthesized as described previously. 10, 12, [19] [20] [21] [22] All of the chemicals were collected for safe disposal discard after use. Anti-Azoxystrobin monoclonal antibody (MoAb), anti-chlorfenapyr MoAb, anti-imazalil MoAb, anti-isoxathion MoAb, and anti-nitenpyram MoAb were provided from Horiba Ltd. (Kyoto, Japan). Anti-boscalid MoAb, which had been prepared in the previous study, was used. 12 Bovine serum albumin (BSA: Prod. No. A7888) was purchased from Sigma-Aldrich Co. (St. Louis, MO). Each of the haptens was conjugated to BSA as described previously. 12 All other chemicals and reagents used were of analytical grade, and purchased from Fujifilm Wako Pure Chemical Co. or Nacalai Tesque, Inc. (Kyoto, Japan).