Np cgg

Manufactured by Thermo Fisher Scientific

Sourced in United States

The NP-CGG is a laboratory equipment product designed for specific applications. It serves a core function within the research and analysis workflow. However, a detailed description that maintains an unbiased and factual approach is not available at this time.

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Lab products found in correlation

Np cgg, by Biosearch Technologies (1 mentions) Imject alum adjuvant, by Thermo Fisher Scientific (1 mentions) Sba clonotyping system hrp kit, by Southern Biotech (1 mentions) Np31 cgg, by Biosearch Technologies (1 mentions) Np 49 ficoll, by Biosearch Technologies (1 mentions) Imject alum, by Thermo Fisher Scientific (1 mentions)

3 protocols using np cgg

Spermidine-Modulated NP-CGG Immunization

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Mice were injected intraperitoneally (ip) with 50 μg NP-CGG (N-5055D-5, Biosearch Technologies) precipitated in Imject Alum adjuvant (Thermo Fisher) on day 0 followed by secondary immunization on or after day 42 (50 μg NP-CGG in PBS ip). Spermidine was given 2 days before the first immunization and then continuously in drinking water. Peripheral blood samples were collected from tail vein on indicated days.

Zhang H., Alsaleh G., Feltham J., Sun Y., Napolitano G., Riffelmacher T., Charles P., Frau L., Hublitz P., Yu Z., Mohammed S., Ballabio A., Balabanov S., Mellor J, & Simon A.K. (2019). Polyamines Control eIF5A Hypusination, TFEB Translation, and Autophagy to Reverse B Cell Senescence. Molecular Cell, 76(1), 110-125.e9.

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B cell response to NP-CGG immunization

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Sh3gl1^−/− and WT littermates were immunized at 8–10 weeks of age by intraperitoneal injection of 50 μg NP‐CGG in alum (Thermo Fisher). Blood samples were taken before immunization and at 7 and 14 days after immunization. Serum NP‐specific antibodies were detected by ELISA on Nunc polysorb plates coated with NP₇ or NP₂₅ for capture. Total serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).

Malinova D., Wasim L., Newman R., Martínez‐Riaño A., Engels N, & Tolar P. (2021). Endophilin A2 regulates B‐cell endocytosis and is required for germinal center and humoral responses. EMBO Reports, 22(9), e51328.

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Induction of TI-2 and TD Immune Responses

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To elicit the TI-2 response, mice were immunized intra-peritoneally with 25 μg of NP-49-Ficoll (F1420, Biosearch Technologies). To elicit the TD response, mice were immunized intra-peritoneally with 50 μg of NP-31-CGG (N50550, Biosearch Technologies) with Imject alum (Thermo Fisher Scientific, Waltham, MA, USA), and boosted with 50 μg of NP-CGG in PBS 8 weeks later. Blood samples were collected on the indicated days after immunization from the tail.

Sasanuma H., Ozawa M, & Yoshida N. (2018). RNA-binding protein Ptbp1 is essential for BCR-mediated antibody production. International Immunology, 31(3), 157-166.

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