Biotin anti gr1 clone rb6 8c5

Manufactured by Thermo Fisher Scientific

Biotin-anti-Gr1 (clone RB6-8C5) is a biotinylated monoclonal antibody that specifically recognizes the Gr-1 antigen, also known as Ly-6G and Ly-6C. It is commonly used in flow cytometry applications to identify and quantify granulocytes, including neutrophils, in mouse samples.

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Close spelling variants of this product

Biotin-coupled mouse anti-Gr1 antibody (Clone RB6-8C5; Anti-Gr1 (RB6-8C5; (clone RB6-8C5, RB6-8C5 Gr1-Biotin

2 protocols using biotin anti gr1 clone rb6 8c5

Immune Cell Isolation and Staining

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Blood cells collected via cardiac puncture were lysed of RBC via incubation with NH₄Cl buffer for 15 min at room temperature with gentle tilting. RBC lysis was inactivated with equal volume of 1xPBS followed by centrifugation at 450 g for 5 min at 4°C and resuspended in 1xPBS. Mammary tissues were digested as previously described until after the RBC lysis step in which mammary cells were subsequently reconstituted in 1xPBS. Staining of cells was carried out with a cocktail of primary antibodies from either BioLegend or eBioscience comprising of APC-Cy7-anti-CD45 (clone 30-F11), FITC-anti-Ly6C (clone HK1.4), PE-anti-Ly6G (clone 1A8), Biotin-anti-Gr1 (clone RB6-8C5), and BV605-anti-CD11b (clone M1/70). Secondary antibody staining was performed with Alexa Fluor 647-streptavidin antibody (BioLegend). Dead cells were stained with eFluor 450-fixable viability dye (eBioscience) before fixation with fixative buffer (BioLegend).

Lim C.L., Or Y.Z., Ong Z., Chung H.H., Hayashi H., Shrestha S., Chiba S., Lin F, & Lin V.C. (2020). Estrogen exacerbates mammary involution through neutrophil-dependent and -independent mechanism. eLife, 9, e57274.

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Multicolor Flow Cytometry Staining

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Staining of cells was carried out with a cocktail of primary antibodies on ice at 1:200 dilution from either BioLegend or eBioscience comprising of APC-Cy7-anti-CD45 (clone 30-F11), FITC-anti-Ly6C (clone HK1.4), PE-anti-Ly6G (clone 1A8), Biotin-anti-Gr1 (clone RB6-8C5), BV605-anti-CD11b (clone M1/70), FITC-anti-TCRγ/δ (clone GL3), PerCP/Cy5.5-anti-CD4 (clone GK1.5), Biotin-anti-CD3e (clone 145-2C11), and BV605anti-CD8a (clone 53-6.7). Secondary antibody staining was performed with Alexa Fluor® 647-streptavidin antibody (BioLegend). Dead cells were stained with eFluor 450-xable viability dye (eBioscience) at 1:1000 dilution before xation with xative buffer (BioLegend).

Lim C.L., & Lin V.C.L. (2021). Estrogen Markedly Reduces Circulating Low-density Neutrophils and Enhances Pro-tumoral Gene Expression in Neutrophil of Tumour-bearing Mice.

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