Cml d4

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The CML-d4 is a laboratory instrument designed for DNA and RNA analysis. It utilizes digital PCR technology to accurately quantify nucleic acid targets. The device enables precise measurement of copy number variations and absolute quantification of gene expression.

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Lab products found in correlation

2 protocols using cml d4

Ginger Powder and Raw Beef Antioxidant Analysis

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The ginger powder and raw beef were purchased from local market (Changsha, China), deep eutectic solvents, including choline chloride, L-carnitine, betaine, glycerol, lactic acid, and xylitol, were purchased by Sigma Chemical Co. (St. Louis, MO, USA). Folin–Ciocalteu’s phenol reagent, rutin, gallic acid, 6-hydroxy-2,5,7,8-tetra-methylchroman-2-carboxylic acid (Trolox), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethyl-benzthiazoline-6-sulfonate) (ABTS), and 2,4,6-tris-(2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). AGEs standards, namely CML, CEL, Nε-(1-carboxymethyl)-L-lysine-d4 (CML-d4), and Nε-(1-carboxyethyl)-L-lysine-d4 (CEL-d4), and HA standards, namely PhIP, IQ, MeIQ, MeIQx, 4,8-DiMeIQx, 7,8-DiMeIQx, IQx, Harmane, and NorHarmane, were supplied by Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Xu Y., Jiao Y., Luo J., He Z., Zeng M., Shen Q., Chen J, & Quan W. (2022). The Influence of Deep Eutectic Solvents Extract from Ginger on the Formation of Heterocyclic Amines and Advanced Glycation End Products in Roast Beef Patties. Foods, 11(20), 3161.

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Quantification of Carbonyl Compounds in Biological Samples

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All chemicals were of analytical grade. CML, CEL, CML-d4, and CEL-d4 were obtained from Santa Cruz Biotechnology (Paso Robles, CA, USA). GO solution (40% in water), MGO solution (40% in water), 3-DG, Pyr, and 2,3-hexanedione (internal standard) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nonafluoropentanoic Acid (NFPA) was obtained from J&K Chemical Co., Ltd. (Shanghai, China). Methanol and acetonitrile of HPLC grade were obtained from TEDIA Co. (Fairfield, OH, USA). All other reagents were obtained from Sinopharm Chemical Reagent Co., Ltd. (China). Ultrapure water was prepared using a Millipore Milli-Q purification system (Bedford, MA, USA).

Xue C., Shi Z., Wang Z., Wang Z., Qin F., Chen J., & Zeng M. (2020). Formation of Three Selected AGEs and their Corresponding Intermediates in Aldose‐ and Ketose‐lysine Systems. eFood, 1(3), 270-278.

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