Double stain hoechst pi kit

Gemcitabine was got from Eli Lilly and Company (Indiana, USA). HSA (20%, 50 mL) was purchased from the Baxter AG (Vienna, Austria). cRGD (sequence: Arg-Gly-Asp-D-Tyr-Lys) was synthesized by Shanghai Gil Biochemistry Co., Ltd. (Shanghai, China); the purity was greater than 99.9%. Sulfo-SMCC (sulfosuccinimidyl-(4-N-maleimidomethyl)cyclohexane-1-carboxylate) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Normal saline (NS) was purchased from Shanghai Baxter Healthcare Co., Ltd. (Shanghai, China). Absolute ethanol and chloroform were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Deionized water was purchased from Fudan University (Shanghai, China). Roswell Park Memorial Institute (RPMI)-1640 culture medium and fetal bovine serum (FBS) were obtained from Gibco (Carlsbad, CA, USA). Double stain Hoechst/PI kit and myristic acid (C-14) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kyushu, Japan). PBS and other reagents were prepared in our laboratory. All the solvents and chemicals were of analytical grade.