Antibiotic antimycotic a a solution

Type A gelatin (300 Bloom), gallic acid (GA), ascorbic acid, hydrogen peroxide, ammonium molybdate, 2,2′-diphenyl-1-picrylhydrazyl (DPPH), matrix metalloproteinase-2 (MMP-2, EC 3.4.24.24), pilocarpine nitrate, and α-chymotrypsin were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-isopropylacrylamide (NIPAAm), from Acros Organics (Geel, Belgium), was purified by recrystallization from n-hexane. Deionized water was filtered using a Milli-Q system (Millipore, Bedford, MA, USA). Balanced salt solution (BSS, pH 7.4) and phosphate-buffered saline (PBS, pH 7.4) were obtained from Alcon (Fort Worth, TX, USA) and Biochrom (Berlin, Germany), respectively. Eagle’s minimum essential medium (MEM) was purchased from Gibco-BRL (Grand Island, NY, USA). Fetal bovine serum (FBS) and the antibiotic/antimycotic (A/A) solution (10,000 U/ml penicillin, 10 mg/ml streptomycin, and 25 μg/ml amphotericin B) were obtained from Biological Industries (Kibbutz Beit Haemek, Israel). All the other chemicals were of reagent grade and used as received without further purification.

Hyaluronic acid sodium salt was obtained from Kewpie (Tokyo, Japan) as a dry powder. It was made by fermentation method and was highly purified. According to information from the supplier, the HA samples used in this study had a weight-average molecular weight of around 1100 kDa. 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride, hyaluronidase type V from sheep testes (1770 units/mg), glucose, and glucose assay kit (glucose oxidase/peroxidase reagent and o-dianisidine reagent) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Balanced salt solution (BSS, pH 7.4) was obtained from Alcon Laboratories (Fort Worth, TX, USA). Phosphate-buffered saline (PBS, pH 7.4) was purchased from Biochrom AG (Berlin, Germany). Medium 199, gentamicin, Hanks’ balanced salt solution (HBSS, pH 7.4), trypsin-ethylenediaminetetraacetic acid (EDTA), and TRIzol reagent were purchased from Gibco-BRL (Grand Island, NY, USA). Collagenase type II was purchased from Worthington Biochemical (Lakewood, NJ, USA). Fetal bovine serum (FBS) and the antibiotic/antimycotic (A/A) solution (10000 U/ml of penicillin, 10 mg/ml of streptomycin, and 25 μg/ml of amphotericin B) were obtained from Biological Industries (Kibbutz Beit Haemek, Israel). All the other chemicals were of reagent grade and used as received without further purification.