Anti vimentin vim

The antibodies included: anti-actin, ab6276 (Abcam, Cambridge, UK); anti-Histone H4 (HIST1H4B), #13919 (Cell Signaling Technology, Danvers, MA, USA); anti- Heat shock cognate 71 kDa protein (HSPA8), #8444 (Cell Signaling Technology); anti-nucleolin (NCL), #12247 (Cell Signaling Technology); anti-nucleophosmin (NPM1), #3542 (Cell Signaling Technology); anti-vimentin (VIM), #3932 (Cell Signaling Technology); anti-plectin-1 (PLEC), #12254 (Cell Signaling Technology). All antibodies were used at the recommended fold dilution described on the manufacture’s datasheet.

Anti-NOSTRIN (Cat no. ab116374) used at 1:100 dilution and anti-Versican/VCAN (Cat no. ab19345) antibody used at dilution 1:1000 was purchased from abcam, USA. Anti-SNAI2/SLUG (Cat no. 9585), anti-SMAD2 (Cat no. 5339), anti-STAT3 (Cat no. 4904), anti-Caldesmon-1/CALD1 (Cat no. 12503), anti-Jagged1/JAG1 (Cat no. 2620), anti-Moesin/MSN (Cat no. 3150), anti-Vimentin/VIM (Cat no. 5741), anti-Integrinα5/ITGα5 (Cat no. 4705), anti-E-cadherin/CDH1 (Cat no. 3195), anti-Integrin-linked protein kinase1/ILK1 (Cat no. 3856), anti-CD44 (Cat no. 5640), anti-EpCAM (Cat no. 36746), anti-CD133 (Cat no. 86781) and anti-GAPDH (Cat no. 2118) antibodies were purchased from Cell Signaling Technology, USA and was used at 1:1000 dilution. Those procured from Santa Cruz Biotechnology, USA were used at 1:250 dilution, which included anti-Collagen type III Alpha 1 chain/COL3A1 (Cat no. sc-514601), anti-Regulator of G-protein signalling 2/RGS2 (Cat no. sc-100761) and anti-Occludin/OCLN (Cat no. sc-133256). anti-Desmoplakin/DSP (Cat no. A303-355A) and anti-Junctional adhesion molecule A/F11R (Cat no. A302-891A) antibodies were from Bethyl lab, USA. HRP-conjugated goat anti-rabbit and goat anti-mouse antibodies purchased from Bethyl lab, USA were used at 1:10000 dilutions.