Anti ha clone 6e2

The following antibodies were used in the present work to characterize PBMC cell populations: anti-human CD16 (clone KD1, Biorad), anti-CD3 (clone CD3-12, Biorad), anti-bovine B cell marker (clone BAQ44A, Kingfisher Biotech), anti-human CD14 (clone TÜK4, Biorad). Anti-FLAG (F7425, Sigma) and anti-HA (clone 6E2, Cell Signaling) antibodies were used to detect protein expression in transfected cells. Anti-GAPDH antibody was used as sample loading control for western blot. Anti-ovine MHC-I antibody (clone 41.17, Biorad) was used as positive control to label cells in antibody dependent cell cytotoxicity assays. Anti-PPRV-N monoclonal antibody was used 1:100 for PPRV detection (Dr. Libeau, CIRAD, Montpellier, France) in flow cytometry experiments. Anti-BTV-VP7 monoclonal antibody (VMRD; CJ-F-BTV-MAB-10 ML) was used for BTV detection. Rat anti-Mouse IgM-FITC (Clone II/41, BD biosciences), anti-mouse IgG-Alexa 488 or 647 (Thermofisher), anti-sheep-IgG Alexa 488 (Thermofisher) were used as secondary antibodies for flow cytometry, and immunofluorescence. Anti-Mouse IgG or anti-Rabbit IgG HRP-conjugated secondary antibodies (GE Healthcare) were used for western blots.

Rabbit polyclonal antibodies specific to mouse CATSPER1^{5 (link)}, EFCAB9^{12 (link)}, and C2CD6^{15 (link)} were described previously. Briefly, to produce antibodies, peptides corresponding to mouse SLCO6C1 (1-14, MAHVRNKKSDDKKA, cysteine added to C-terminus) (GenScript) were synthesized and conjugated to KLH carrier protein. Antisera from the immunized rabbits were affinity-purified using the peptide immobilized on Sulfo Link Plus resin (Pierce). Other antibodies used in this study are commercially available as follows: TRIM69, Origene; FANCM, Affinity Biosciences; acetylated tubulin, Sigma; anti-HA (clone 6E2), Cell Signaling Technology. All the chemicals were from Sigma Aldrich unless otherwise indicated.