Previously characterized CII T-cell epitopes were synthesized in a sequence derived from the native murine collagen II (Col2A1) gene cDNA sequence by Genescript (Piscataway, NJ). The resulting construct was cloned via Gibson assembly (New England Biolabs, Rowley, MA) into either pAAV-MCS or pacAd5 CMV K-N (all from Cell Biolabs, San Diego, CA) to generate the pAAV-MCS: CII or pacAd5 CMV K-N: CII constructs.
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