Dc protein assay 500 0119

The DC Protein Assay (500-0119) and Clarity Western ECL Substrate (170-5061) were from BioRad. The Effectene Transfection Reagent (301427) was from Qiagen. The Lipofectamine RNAiMAX Reagent (100014472) was from Thermo Fisher Scientific. ProLong Diamond antifade mountant without (P36970) or with (P36971) DAPI (4′,6-diamidino-2-phenylindole) was from Thermo Fisher Scientific. BafA1 (11038) and MRT68921 (1990520000040) were from Cayman Chem. PIK-III was from Selleckchem (S7683). Bort (B-1408) was from LC Laboratories. Janelia Fluor 646 HaloTag Ligand (GA1120) was from Promega.
Plasmids used and sources are given in Table 1. Antibodies used along with hosts and manufacturers appear in Table 2.

Reagents and antibodies were purchased from the following suppliers: HRP–conjugated goat anti-mouse IgG (31430) and goat anti-rabbit IgG (31461); Alex Fluor 488 goat anti-mouse IgG (A11029), Texas Red goat anti-mouse IgG (T-862), and goat anti-rabbit IgG (T-2767); and Alexa Fluor Plus 647 goat anti-mouse IgG (A32728) and goat anti-rabbit IgG (A32733) are from Thermo Fisher Scientific. Anti-beclin-1 (NB500-249), anti-FAM134B (NBP2-55248), and anti-Sec62 (NBP1-84045) are from Novus Biologicals. Anti-calnexin (C4731), anti-HA (H9658), anti-LC3B (L7543), anti-Myc (C3956), and anti-tubulin (T-9026) are from Sigma-Aldrich. Anti-ERGIC53 (ALX-804-602) is from Enzo Life Sciences. Anti-GABARAP+L1+L2 (ab109364) and anti-GM130 (ab52649) are from Abcam. Anti-LAMP2 (555803) and anti-Sec31A (612351) are from BD Pharmingen. Anti-p62 (H00008878-M0162) is from Abnova. Anti-VMP1 (12978s) is from Cell Signaling. DC Protein Assay (500-0119) and Clarity Western ECL Substrate (170-5061) are from Bio-Rad. Effectene Transfection Reagent (301427) is from Qiagen. Lipofectamine RNAiMAX Reagent (100014472) is from Thermo Fisher Scientific. MTS cross-linking reagents M3M (1,3-propanediyl bis-MTS) and M8M (1,5-pentanediyl bis-MTS) are from Toronto Research Chemicals. ProLong Diamond antifade mountant without (P36970) or with DAPI (P36971) is from Thermo Fisher Scientific.