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Goat anti trop2 apc

Manufactured by R&D Systems
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Goat anti-Trop2-APC is a lab equipment product that detects the Trop2 protein. It is labeled with the fluorescent dye APC, which allows for detection and analysis using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

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Close spelling variants of this product

Anti-Trop2 (4 citations) Trop2 (4 citations)

5 protocols using goat anti trop2 apc

1

Cell Sorting and Antibody Staining

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Dissociated cells were stained with directly conjugated primary antibodies: rat anti-CD49f-PE (BioLegend), rat anti-CD326 (EpCAM)-APC/Cy7 (BioLegend), goat anti-Trop2-APC (R &D Systems), rat anti-CD31-FITC (BioLegend), rat anti-CD45-FITC (BioLegend), and rat anti-Ter119-FITC (BioLegend) for 20 min on ice. Rat anti-ESAM-FITC (BioLegend) was also added to the Lin panel for some experiments. Rat anti-CD44-FITC (BioLegend) was used for analysis. Cells were stained in media containing RPM11640 (GIBCO), 10% FBS (Corning), 1x penicillin-streptomycin (GIBCO), and 10uM of the p160ROCK inhibitor Y-27632 dihydrochloride (Tocris Bioscience). Sorting was performed on a BD FACS Aria II (BD Biosciences) and flow cytometry analysis was performed on a BD FACS Canto (BD Biosciences).
Crowell P.D., Fox J.J., Hashimoto T., Diaz J.A., Navarro H.I., Henry G.H., Feldmar B.A., Lowe M.G., Garcia A.J., Wu Y.E., Sajed D.P., Strand D.W, & Goldstein A.S. (2019). Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell reports, 28(6), 1499-1510.e6.
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2

Multicolor Flow Cytometry of Mouse Prostate Cells

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Dissociated cells from mouse prostate were stained with rat anti-CD49f-PE (BioLegend), rat anti-CD326 (EpCAM)-APC/Cy7 (BioLegend) and goat anti-Trop2-APC (R & D Systems) for 20 min on ice. Cells were washed with PBS and fixed in 1ml of 2% paraformaldehyde made from 16% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 min on ice. Cells were washed with PBS and permeabilized in 1ml of permeabilization buffer (0.1% Saponin (Sigma-Aldrich), 5% FBS (Corning) in PBS) for 15 min at room temperature in the dark. Cells were resuspended in 100 μL of permeabilization buffer and stained with either rabbit anti-cytokeratin 5-Alexa Fluor 647 (Abcam) and rabbit anti-cytokeratin 8-Alexa Fluor 488 (Abcam), mouse anti-cytokeratin 14-FITC (Abcam), mouse anti-cytokeratin 18-FITC (Abcam) or rat anti-Ki67-FITC (BioLegend) for 20 min at room temperature in the dark. Cells were washed and resuspended in permeabilization buffer for analysis on a BD FACS Canto (BD Biosciences).
Crowell P.D., Fox J.J., Hashimoto T., Diaz J.A., Navarro H.I., Henry G.H., Feldmar B.A., Lowe M.G., Garcia A.J., Wu Y.E., Sajed D.P., Strand D.W, & Goldstein A.S. (2019). Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell reports, 28(6), 1499-1510.e6.
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3

Cell Sorting and Antibody Staining

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Dissociated cells were stained with directly conjugated primary antibodies: rat anti-CD49f-PE (BioLegend), rat anti-CD326 (EpCAM)-APC/Cy7 (BioLegend), goat anti-Trop2-APC (R &D Systems), rat anti-CD31-FITC (BioLegend), rat anti-CD45-FITC (BioLegend), and rat anti-Ter119-FITC (BioLegend) for 20 min on ice. Rat anti-ESAM-FITC (BioLegend) was also added to the Lin panel for some experiments. Rat anti-CD44-FITC (BioLegend) was used for analysis. Cells were stained in media containing RPM11640 (GIBCO), 10% FBS (Corning), 1x penicillin-streptomycin (GIBCO), and 10uM of the p160ROCK inhibitor Y-27632 dihydrochloride (Tocris Bioscience). Sorting was performed on a BD FACS Aria II (BD Biosciences) and flow cytometry analysis was performed on a BD FACS Canto (BD Biosciences).
Crowell P.D., Fox J.J., Hashimoto T., Diaz J.A., Navarro H.I., Henry G.H., Feldmar B.A., Lowe M.G., Garcia A.J., Wu Y.E., Sajed D.P., Strand D.W, & Goldstein A.S. (2019). Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell reports, 28(6), 1499-1510.e6.
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4

Multicolor Flow Cytometry of Mouse Prostate Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dissociated cells from mouse prostate were stained with rat anti-CD49f-PE (BioLegend), rat anti-CD326 (EpCAM)-APC/Cy7 (BioLegend) and goat anti-Trop2-APC (R & D Systems) for 20 min on ice. Cells were washed with PBS and fixed in 1ml of 2% paraformaldehyde made from 16% paraformaldehyde (Electron Microscopy Sciences) in PBS for 15 min on ice. Cells were washed with PBS and permeabilized in 1ml of permeabilization buffer (0.1% Saponin (Sigma-Aldrich), 5% FBS (Corning) in PBS) for 15 min at room temperature in the dark. Cells were resuspended in 100 μL of permeabilization buffer and stained with either rabbit anti-cytokeratin 5-Alexa Fluor 647 (Abcam) and rabbit anti-cytokeratin 8-Alexa Fluor 488 (Abcam), mouse anti-cytokeratin 14-FITC (Abcam), mouse anti-cytokeratin 18-FITC (Abcam) or rat anti-Ki67-FITC (BioLegend) for 20 min at room temperature in the dark. Cells were washed and resuspended in permeabilization buffer for analysis on a BD FACS Canto (BD Biosciences).
Crowell P.D., Fox J.J., Hashimoto T., Diaz J.A., Navarro H.I., Henry G.H., Feldmar B.A., Lowe M.G., Garcia A.J., Wu Y.E., Sajed D.P., Strand D.W, & Goldstein A.S. (2019). Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell reports, 28(6), 1499-1510.e6.
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5

Prostate Cell Immunophenotyping by Flow

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Single-cell suspensions of 5 × 104–1 × 106 cells from mouse prostate tissue of different ages (each n = 4) were stained in cell staining buffer comprised of 1X DPBS (Gibco) with 5 g/L protease-free bovine serum albumin (Sigma-Aldrich) and 200 mg/L sodium azide (Sigma-Aldrich). Nonspecific antibody binding by Fc receptors was blocked using TruStain fcX (anti-mouse CD16/32) Antibody (BioLegend) according to the manufacturer’s protocol. Cells were then stained with rat anti-CD49f-PE (BioLegend), rat anti-CD326 (EpCAM)-APC/Cy7 (BioLegend), goat anti-Trop2-APC (R&D Systems), and rat anti-CD45-FITC (BioLegend) for 30 minutes at room temperature. Cells were washed with cell staining buffer then fixed in 1% paraformaldehyde (Electron Microscopy Sciences) for 10–15 minutes at 37°C. After fixation, cells were chilled on ice for 1 minute, washed with cell staining buffer, and stored at 4°C before analysis on a FACSCanto flow cytometer (BD Biosciences).
Fox J.J., Hashimoto T., Navarro H.I., Garcia A.J., Shou B.L, & Goldstein A.S. (2023). Highly multiplexed immune profiling throughout adulthood reveals kinetics of lymphocyte infiltration in the aging mouse prostate. Aging (Albany NY), 15(9), 3356-3380.
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