Ivermectin (I141334), IR780 (207399-07-3), 6-aminohexanoic acid (A306000), triethylamine (T103285), 4-dimethylaminopyridine (DMAP) (D109207), 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) (E106172) and 1,3-Diphenylisobenzofuran (DPBF) were purchased from Aladdin Industrial Corporation. Hydroxychloroquine (BD134189) was purchased. Hyaluronic acid (MB7264) was purchased from Meilunbio. Dulbecco’s modified Eagle’s medium (DMEM), RPMI1640, was purchased from Thermo Fisher Science. Dimethyl sulfoxide (DMSO) crystal violet was purchased from Micros Sigma. Fetal bovine serum albumin (FBS) was provided by Biowest. Annexin V-FITC/PI cell apoptosis detection kit was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. LDH release detection kit was provided by Beyotime. 2’,7’-dichlorofluorescein diacetate (DCFH-DA) was purchased from Sigma-Aldrich.
Annexin 5 fitc pi cell apoptosis detection kit
Manufactured by Yeasen
Sourced in China
The Annexin V-FITC/PI cell apoptosis detection kit is a laboratory equipment used to detect and quantify apoptosis in cell populations. It utilizes Annexin V, a calcium-dependent phospholipid-binding protein, conjugated to the fluorescent dye FITC, and the DNA-binding dye propidium iodide (PI) to distinguish between viable, early apoptotic, and late apoptotic/necrotic cells.
Automatically generated - may contain errors
2 protocols using annexin 5 fitc pi cell apoptosis detection kit
1
Synthesis and Evaluation of Photosensitizers
2
Quantifying Lung Cancer Cell Apoptosis
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Lung cancer cells transfected with hsa_circ_0046264 were collected and treated with Annexin V-FITC/PI Cell Apoptosis Detection Kit (YEASEN, Shanghai, China) in accordance with the manufacturer’s instructions. Cell apoptosis was then detected by flow cytometry (FCM) FACS Calibur (BD Biosciences, CA, USA) and analyzed by software FACS Diva.
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