Anti cleaved caspase 9 asp315

Mouse monoclonal anti-PARP (1:500), anti-beclin-1 (1:500), rabbit polyclonal anti-Bax (1:2000), and anti-Bak (1:3000) were from BD Biosciences (San Jose, CA, USA); mouse monoclonal anti-Bcl-xL (1:1000), rabbit polyclonal anti-Mcl-1 (1:1000), anti-cyclin B1 (1:500), and anti-p-histone H3 (Ser10) (1:1000) were from Santa Cruz (Santa Cruz, CA, USA); mouse monoclonal anti-β-actin (1:10,000), rabbit polyclonal anti-LC3B (1:2000), and anti-p62 (1:2000) were from Sigma; rabbit polyclonal anti-cleaved caspase-9 (Asp315) (1:500) and anti-cleaved caspase-3 (Asp175) (1:500) were from Cell Signaling (Danvers, MA, USA).

The antibodies and dilutions used for western blots were as follows: rabbit polyclonal anti-FBXW7 (1:500) and anti-AURKA (1:2,000) from Novus; rabbit polyclonal anti-MCL1 (S-19, 1:1,000), anti-Cyclin B1 (1:1,000), anti-phospho-histone H3 (Ser10, 1:500) and mouse monoclonal anti-PTTG1 (1:1,000) from Santa Cruz; mouse monoclonal anti-β-actin (Ac15, 1:20,000) and rabbit polyclonal anti-c-Myc (1:1,000) from Sigma; rabbit polyclonal anti-cleaved caspase-9 (Asp 315, 1:500), anti-cleaved caspase-3 (Asp 175, 1:500) from Cell Signaling; rabbit polyclonal anti-BubR1 (1:3,000) from Bethyl; mouse monoclonal anti-PARP (1:500) from BD Bioscience; mouse monoclonal anti-Cyclin E (1:500) from Monosan; mouse monoclonal anti-PLK1 (1:10,000) from Millipore, and rat monoclonal anti-HA-peroxidase (50 mU/mL) from Roche. For immunohistochemical analyses, anti- FBXW7 (1:2,000), anti- Cyclin E (1:100), anti- MCL1 (1:1,250), anti-AURKA (1:700), and anti-PLK1 (1:1,000) were used.

Bilirubin (B4126-5G) was purchased from Sigma-Aldrich (USA). Vemurafenib (S1267), KO-947 (S8569), and LY3214996 (S8534) were obtained from Selleckchem (Houston, TX). Antibodies, including anti-PARP (#9532), Caspase 3 (#9662), anti-cleaved Caspase-3 (Asp175) (#9661), anti-Caspase 9 (#9502), anti-cleaved Caspase-9 (Asp315) (#9505), anti-phospho-B-Raf (Ser445) (#2696), anti-B-Raf (#14814), anti-phospho-MEK1/2 (Ser217/221) (#3958), anti-MEK1/2 (#4694), anti-phospho-ERK1/2 (Thr202/Tyr204) (#4370), anti-ERK1/2 (#4695), anti-phospho-MNK1 (Thr197/202) (#2111), anti-MNK1 (#2195), anti-phospho-eIF4E (Ser209) (#9741), anti-eIF4E (#2067), anti-phospho-p70S6K (Thr421/Ser424) (#9204), anti-p70S6K (#2708), anti-phospho-4eBP1(Thr37/46) (#2855), anti-4eBP1 (#9644), and anti-GAPDH (#5174) were purchased from Cell Signaling Technology (Beverly, MA).