After primary hepatocytes were treated with OA, OA+BBR and OA+ROT for 24 h, we collected the supernatant of each group and assessed the non-esterified free fatty acids (Wako). And the results were normalized to the NEFA amounts of OA-treated blank well (cell free) to evaluate the fatty acid uptake with a colorimetric assay.
For cellular triglyceride assay, we incubated primary hepatocytes with different treatment for 24 h, followed by lysing the cells in 5 % NP-40. The samples were heated to 95 °C for 5 min and cooled down to room temperature. After being centrifuged, the collected supernatant was used to determine the triglyceride level (BioVision).
For cellular triglyceride assay, we incubated primary hepatocytes with different treatment for 24 h, followed by lysing the cells in 5 % NP-40. The samples were heated to 95 °C for 5 min and cooled down to room temperature. After being centrifuged, the collected supernatant was used to determine the triglyceride level (BioVision).