Superfrost plus glass slides

Manufactured by Carl Roth

Sourced in Germany

SuperFrost Plus glass slides are standard microscope slides designed for various laboratory applications. They feature a coated surface that enhances cell and tissue adhesion, providing a reliable substrate for microscopic analysis and sample preparation.

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Lab products found in correlation

Cm3050, by Leica (2 mentions) Tissue tek, by Sakura Finetek (2 mentions) Tissue tek oct, by Sakura Finetek (1 mentions) Cyclone plus storage phosphor system, by PerkinElmer (1 mentions) 2 methylbutane, by Carl Roth (1 mentions)

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Glass slides (12 citations) SuperFrost Plus slides (3 citations) Superfrost glass slides (2 citations) SuperFrost slides (2 citations)

5 protocols using superfrost plus glass slides

Cerebellar Tissue Sectioning and Preservation

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Brains were dissected out following cervical dislocation, fresh frozen in powdered dry ice.
Sagittal cerebellar sections (formalin fixed: 12 µm thick; fresh-frozen: 30µm thick) were cut in a cryostat and collected on SuperFrost Plus glass slides (Roth, France) and stored at -80°C. Tissue sections were postfixed in acetone-methanol (1:1, -20°C, 5 min) before immunochemistry, as previously described (Daoud et al., 2009b; Vaillend et al., 2010) (link).

Belmaati Cherkaoui M., Vacca O., Izabelle C., Boulay A.C., Boulogne C., Gillet C., Barnier J.V., Rendon A., Cohen-Salmon M, & Vaillend C. (2021). Dp71 contribution to the molecular scaffold anchoring aquaporine-4 channels in brain macroglial cells. Glia, 69(4).

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Ex Vivo Pancreas Autoradiography in Mice

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Mice were anesthetized with isoflurane and buprenorphine injected subcutaneously (Temgesic, 0.1 mg/kg body weight). Following ^99mTC-Nanocoll SPECT/CT imaging, ex vivo autoradiography of pancreas was performed. A blood sample was collected by cardiac puncture, and mice were euthanized by blood loss under anesthesia. Subsequently, pancreas was removed, embedded in Tissue-Tek® O.C.T. (Sakura-Finetek, USA), and frozen on dry ice. Sections (−20°C, 20 μm thick) were prepared by a cryostat and thaw-mounted on Superfrost^® Plus glass slides (Carl Roth GmbH & Co. KG, Karlsruhe, Germany). Sections were thawed and dried for 30 minutes at 22°C. Slides were exposed to a phosphor storage screen inside a cassette overnight at 21°C. Images were developed using a Cyclone Plus storage phosphor system (Perkin Elmer, MA, USA), and the results were evaluated using Image J software (National Institutes of Health, MD, USA). A calibration factor was calculated after counting the radioactivity in the blood samples and normalizing to injection dose per animal weight. Image pixel values were converted to SUV.

Dorraji E.S., Oteiza A., Kuttner S., Martin-Armas M., Kanapathippillai P., Garbarino S., Kalda G., Scussolini M., Piana M, & Fenton K.A. (2021). Positron emission tomography and single photon emission computed tomography imaging of tertiary lymphoid structures during the development of lupus nephritis. International Journal of Immunopathology and Pharmacology, 35, 20587384211033683.

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Autoradiography of Muscarinic Receptor Subtypes

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For receptor determination, autoradiography was performed in several brain areas [motor cortex (MOCx), somatosensory cortex (SSCx), visual cortex (VisCx), striatum (Caudatum-Putamen, CPu), nucleus accumbens (NAc), thalamus (TH), hippocampus (Hipp) and its specific areas CA1, CA3 and dentate gyrus (DG), olfactory tubercle (OT), pons (Pons), and medulla oblongata (MY)] on sagittal brain sections of M₁KO, M₂KO, M₄KO mice and their WT littermates. Brains were rapidly removed (4–6 brains per group), frozen on dry ice, and then stored at −80°C until cryostat sectioning. Sixteen-micrometer thick sagittal sections were cut on a cryostat at −20°C and thaw-mounted on Superfrost® Plus glass slides (Carl Roth GmbH & Co. KG, Karlsruhe, Germany) and stored in storage boxes at −80°C until use. For autoradiography experiments brain sections were allowed to thaw and dry for 30 min at 22°C.

Valuskova P., Farar V., Forczek S., Krizova I, & Myslivecek J. (2018). Autoradiography of 3H-pirenzepine and 3H-AFDX-384 in Mouse Brain Regions: Possible Insights into M1, M2, and M4 Muscarinic Receptors Distribution. Frontiers in Pharmacology, 9, 124.

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Spinal Cord Tissue Preparation and Immunohistochemistry

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Mice were anesthetized by intraperitoneal injection of 16% sodium pentobarbital solution (Narcoren, Merial, Hallbergmoos, Germany, 5 µl/g body weight) and were transcardially perfused with 4% formaldehyde in 0.1 M sodium cacodylate buffer, pH 7.3. The spinal cord was removed two hours after fixing, post-fixed overnight at 4 °C and then immersed in 15% sucrose solution in 0.1 M cacodylate buffer, pH 7.3, for 1 day at 4 °C. Afterwards the tissue was frozen for 2 min in 2-methyl-butane (isopentane, Carl Roth, Karlsruhe, Germany) pre-cooled to −80 °C. For sectioning, the spinal cord segment was attached to a cryostat specimen holder using TissueTek (Sakura Finetek Europe, Zoeterwoude, The Netherlands). Serial transverse or parasagittal sections of 25 μm thickness were cut on a cryostat (Leica CM3050, Leica Instruments, Nußloch, Germany) and picked up on Super Frost Plus glass slides (Roth, Karlsruhe, Germany). Sampling of sections was always done in a standard sequence so that four sections 250 μm apart were present on each slide. Immunohistochemistry was performed as described earlier61 (link).

Saini V., Lutz D., Kataria H., Kaur G., Schachner M, & Loers G. (2016). The polysialic acid mimetics 5-nonyloxytryptamine and vinorelbine facilitate nervous system repair. Scientific Reports, 6, 26927.

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Spinal Cord Injury Mouse Model

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At 12 weeks after spinal cord injury, mice were anesthetized with 16% solution of sodium pentobarbital (Narcoren, Merial, Hallbergmoos, Germany, 5 μL/g body weight). The animals were transcardially perfused with fixative consisting of 4% formaldehyde and 0.1% CaCl₂ in 0.1 mol/L cacodylate buffer, pH 7.3, for 15 minutes at room temperature. Following perfusion, the spinal cords were left in situ for 2 hours at room temperature, after which they were dissected out and post-fixed overnight (18–22 hours) at 4°C in the same solution used for perfusion. Tissue was then immersed into 15% sucrose solution in 0.1 mol/L cacodylate buffer, pH 7.3, for 2 days at 4°C, embedded in Tissue Tek (Sakura Finetek, Zoeterwoude, NL, USA), and frozen by 2-minute immersion into 2-methyl-butane (isopentane) precooled to −80°C. Serial transverse or longitudinal sections were cut using a cryostat (Leica CM3050, Leica Instruments, Nußloch, Germany). Sections, 25-μm-thick, were collected on SuperFrost Plus glass slides (Roth, Karlsruhe, Germany). Sampling of sections was always done in a standard sequence so that six sections 250 μm apart were present on each slide.

Aleksić D., Aksić M., Divac N., Radonjić V., Filipović B, & Jakovčevski I. (2014). Thermomineral water promotes axonal sprouting but does not reduce glial scar formation in a mouse model of spinal cord injury. Neural Regeneration Research, 9(24), 2174-2181.

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