Mini protean tetra gel cell

20 μg of protein samples were mixed with 2× loading buffer [38 (link)] and heated to 95°C for 6 min. A protein ladder (PageRuler unstained protein ladder, Thermo Fischer Scientific Inc., Waltham, MA, USA) and the protein sample were loaded on a SDS mini gel containing 12% acrylamide resolving gel and 5% stacking gel prepared according to [39 ]. The gel was separated in a Mini-Protean^® Tetra gel cell (Bio-Rad, Herculas, CA, USA) by applying 80 V for 30min followed by 180 V for 65min. A fluorescent picture was taken at 312nm irradiation using a UV transilluminator (UV star, Bio-Rad), a PowerShot A640 camera (Canon, Tokyo, Japan) and a 520 nm long pass filter. The gel was stained with RAPIDstain™ (G-Biosciences, St. Louis, MO, USA).

Aliquots (10 µL) of each pre-incubated catalase (9 µL of untreated catalase and 1 µL of deionized water) were mixed with 10 µL of 2× loading buffer [44 (link)] and heated to 95 °C for 6 min. A protein ladder (PageRuler unstained protein ladder, Thermo Scientific) and 15 µL of each sample were loaded on a 12% SDS mini gel and separated in a Mini-Protean^® Tetra gel cell (Bio-Rad, Herculas, CA, USA) by applying 80 V for 30 min followed by 180 V for 65 min. A fluorescent picture was taken at 365 nm irradiation using a UV transilluminator (Bio-Rad, UV star), a PowerShot A640 camera (Canon, Tokyo, Japan) and a commercially available UV filter (HMC Hoya Multi-Coated Filter, Hoya, Tokyo, Japan). The gel was stained with RAPIDstain™ (G-Biosciences, St. Louis, MO, USA).