Imaging with 99mTc-pyrophosphate was performed according to one of two protocols. All subjects underwent 99mTc-PYP cardiac imaging on either a Ventri (GE, Boston, USA, n=16) or GE NM Discovery 670 (GE, Boston, USA, n=108) scanner. Patients received 740 or 770 Mbq (20 or 21 mCi) of 99mTc-PYP intravenously and planar images were obtained at 1-hour and 3-hours post injection over 5–8 minutes duration, with a heart-centered field of view. SPECT images were acquired at 3-hours in all patients. Patients imaged with the Ventri camera system had tomogram images acquired over 60 steps, with a total acquisition time of 12 minutes using a low-energy high-resolution collimator, a 10% energy window and a 64 × 64 matrix. Patients imaged with the GE NM Discovery 670 had tomogram images acquired over 15 minutes using a low-energy high-resolution collimator, a 10% energy window and a 64 × 64 matrix. All images were reconstructed with filtered back projection.
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