Anti mouse hrp na 931

Manufactured by Cytiva

Anti-mouse-HRP (NA 931) is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to mouse primary antibodies in immunoassays and other immunochemical techniques.

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Lab products found in correlation

Anti goat hrp sc 2020, by Santa Cruz Biotechnology (1 mentions) Ha irdye800, by Rockland Immunochemicals (1 mentions) Pentr vector, by Thermo Fisher Scientific (1 mentions) Ha peroxidase, by Merck Group (1 mentions) Mip18 fam96b 20108 1 ap, by Proteintech (1 mentions) Mms19 16015 1 ap, by Proteintech (1 mentions) Ab96488, by Abcam (1 mentions) Ab83088, by Abcam (1 mentions) Fusionsolo, by WITec (1 mentions)

2 protocols using anti mouse hrp na 931

Co-Immunoprecipitation of Protein Interactors

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AMOTL1 (HPA001196), Flag (M2), FAT1 (HPA001869) and HA-peroxidase antibodies were obtained from Sigma. Merlin (E-2 and A-19), RNA Pol2 (N-20), SPT16 (H-4), HA (Y-11), MYC (A-14) and anti-goat-HRP (sc-2020) antibodies from Santa Cruz and p21 (05–345) from Millipore. AMOT (303A), CDC73 (170A), CHD1 (218A), CTR9 (395A), DDB1 (462A), LEO1 (175A), Merlin iso1 (A578), Merlin iso2 (A579), PAF1 (172A), PELP1 (180A), RTF1 (179A), YAP (309A), SF3A3 (507A), SF3B1 (997A), SF3B3 (508A), TAT-SF1 (023A), VPRBP (887A and 888A), FAT1 (402A and 403A) antibodies were from Bethyl Laboratories, FLAG-Dylight680 and HA-IRDye800 from rockland. Anti-mouse-HRP (NA 931) and anti-rabbit-HRP (NA934) were obtained from Amersham. cDNAs were cloned into the pENTR vector (Invitrogen) and transferred to Gateway-compatible expression vectors using recombination-mediated Gateway technology (Invitrogen). Mutations were generated using the QuikChange site-directed mutagenesis kit (Stratagene).

Roehrig A.E., Klupsch K., Oses-Prieto J.A., Chaib S., Henderson S., Emmett W., Young L.C., Surinova S., Blees A., Pfeiffer A., Tijani M., Brunk F., Hartig N., Muñoz-Alegre M., Hergovich A., Jennings B.H., Burlingame A.L, & Rodriguez-Viciana P. (2021). Cell-cell adhesion regulates Merlin/NF2 interaction with the PAF complex. PLoS ONE, 16(8), e0254697.

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Western Blot Antibody Validation Protocol

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Western blots were imaged on a FUSION SOLO (Witec AG) chemiluminescence imaging system. The following primary antibodies (at a dilution of 1:1000) were used: β-actin-horseradish peroxidase (HRP) (C4, sc-47778; Santa Cruz Biotechnology), DNA2 (ab96488; Abcam), CIAO1 (ab83088; Abcam), MIP18/FAM96b (20108-1-AP; Proteintech), MMS19 (16015-1-AP; Proteintech). Secondary antibodies (at a dilution of 1: 2500) were anti-Mouse-HRP (NA931; Amersham) and anti-Rabbit-HRP (NA934; Amersham). Uncropped blots can be found in Supplementary Fig. 6.

Mariotti L., Wild S., Brunoldi G., Piceni A., Ceppi I., Kummer S., Lutz R.E., Cejka P, & Gari K. (2020). The iron–sulphur cluster in human DNA2 is required for all biochemical activities of DNA2. Communications Biology, 3, 322.

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