Oregon green bapta 5n

Manufactured by Thermo Fisher Scientific

Sourced in Switzerland, United States

Oregon Green BAPTA-5N is a fluorescent calcium indicator dye. It is used to detect and measure intracellular calcium levels in biological samples.

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Lab products found in correlation

Liberase blendzyme 3, by Roche (1 mentions) Glycine, by Fujifilm (1 mentions) Urea, by Fujifilm (1 mentions) Sucrose, by Fujifilm (1 mentions) Ethanol, by Dojindo Laboratories (1 mentions) Potassium chloride (kcl), by Fujifilm (1 mentions) Cacl2, by Fujifilm (1 mentions) Sodium phosphate, by Fujifilm (1 mentions) Calcein, by Merck Group (1 mentions) Ethylenediamine n n n n tetraacetic acid, by Dojindo Laboratories (1 mentions) 2 4 2 hydroxyethyl 1 piperazinyl ethanesulfonic acid hepes, by Dojindo Laboratories (1 mentions) N octyl β d glucoside, by Dojindo Laboratories (1 mentions) Polyethylene glycol mono p isooctylphenyl ether triton x 100, by Nacalai Tesque (1 mentions) Hexapotassium salt, by Thermo Fisher Scientific (1 mentions) Sodium chloride (nacl), by Fujifilm (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (1 mentions) Optoled, by Cairn Research (1 mentions)

3 protocols using oregon green bapta 5n

Protocols for Cardiac Cell Experiments

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Krebs-Henseleit (K-H) solution (in mmol/l): 118 NaCl, 4.7 KCl, 1.66 MgCl₂, 2 CaCl₂, 1.18 KH₂PO₄, 15 NaHCO₃, 11 glucose, pH 7.35, saturated with 95% O₂ and 5% CO₂.
Tyrode solution (in mmol/l): 135 NaCl, 5.4 KCl, 5 MgCl₂, 1 CaCl₂, 0.33 NaH₂PO₄, 10 HEPES, pH 7.3.
Ca-free Tyrode solution (in mmol/l): 135 NaCl, 5.4 KCl, 5 MgCl₂, 0.02 CaCl₂, 0.33 NaH₂PO₄, 10 HEPES, pH 7.3.
Enzyme solution (in mmol/l): 135 NaCl, 5.4 KCl, 5 MgCl₂, 0.02 CaCl₂, 0.33 NaH₂PO₄, 10 HEPES, 0.19 U ml⁻¹ Liberase Blendzyme 3 (Roche Diagnostics, Basel, Switzerland), pH 7.3.
Storage solution (in mmol/l): 106 CH₃SO₃H, 106 KOH, 3.9 KCl, 2.4 MgSO₄, 8 K₂HPO₄, 1 ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA), 22 taurine, 22 glucose, pH 7.3.
Cacodylate buffer (in mmol/l): 150 Na cacodylate, 2 CaCl₂, pH 7.3.
External solution (in mmol/l): 135 NaCl, 5.4 CsCl, 10 HEPES, 5 MgCl₂, 0.33 NaH₂PO₄, 1 CaCl₂, 0.01 IBMX, 0.02 TTX, pH 7.3.
Internal solution (in mmol/l): 135 CsCH₃SO₃, 10 CsCl, 10 HEPES, 2 EGTA, 2 CaEGTA, 3 MgSO₄, 3 ATPNa₂, 1 Oregon Green BAPTA-5N (Thermo Fisher Scientific, Brno, Czech Republic), 0.05 cAMP, pH 7.3; 100 nM free [Ca²⁺].
Chemicals were from Sigma-Aldrich (USA), if not stated otherwise.

Novotová M., Zahradníková A J.r., Nichtová Z., Kováč R., Kráľová E., Stankovičová T., Zahradníková A, & Zahradník I. (2020). Structural variability of dyads relates to calcium release in rat ventricular myocytes. Scientific Reports, 10, 8076.

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Characterization of Membrane Proteins

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N-Octyl-β-d-glucoside (βOG),
ethylenediamine-N-N-N′-N′-tetraacetic
acid (EDTA), and 2-[4-(2-hydroxyethyl)-1-piperazinyl] ethanesulfonic
acid (HEPES) were purchased from Dojindo Laboratories (Kumamoto, Japan).
Ethanol, isopropyl-β-d(−)-galactopyranoside
(IPTG), 2-amino-2-hydroxymethyl-1,3-propanediol (Tris), sodium phosphate,
lysozyme, sucrose, glucose, urea, glycine, NaCl, NaF, KCl, and CaCl₂ were purchased from Wako Pure Chemical Industries, Ltd. (Osaka,
Japan). Polyethylene glycol mono-p-isooctylphenyl
ether (Triton X-100) was purchased from Nacalai Tesque Inc., (Kyoto,
Japan). 1,2-Dioleoyl-sn-glycero-3-phosphocholine
(DOPC) and 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (DOPG) were purchased from Avanti
Polar Lipids Inc. (Alabaster, AL, USA). Alexa Fluor 546 carboxylic
acid, succinimidyl ester, Oregon Green BAPTA-5N, and hexapotassium
salt were purchased from Thermo Fisher Scientific (Waltham, MA, USA).
Calcein was purchased from Sigma-Aldrich (St. Louis, MO, USA). 5-Carboxyfluorescein
was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).

Tosaka T, & Kamiya K. (2024). Formation of Cell-Sized Liposomes Incorporating a β-Barrel-Structured Porin through Rehydration of a Phospholipid-Membrane Protein Dried Film. ACS Omega, 9(5), 5911-5918.

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Calcium Imaging of Axonal Varicosities

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Epifluorescence imaging was performed with two LEDs on a dual lamp-house controlled by an OptoLed (Cairn Research). The first LED excited the sample at 470 ± 40 nm and the second LED at 572 ± 35 nm. Emitted light (520 ± 40 nm and 630 ± 60 nm) was detected with an electron-multiplying charge-coupled device camera (Ixon; Andor). Filters were from Chroma Technology. For calcium imaging, the calcium indicator Oregon Green BAPTA-5N (OGB5) was included in the presynaptic IS at a final concentration of 500 µM and Oregon Green BAPTA-1 (OGB1) at a final concentration of 100 µM (both from Thermo Fisher Scientific). After an axonal varicosity was identified, it was positioned on the center of the camera chip by moving the preparation, and fluorescent images were acquired at a frame rate of 4.5 ms (218 Hz), in 2 × 2 binned pixels, with Andor Solis software. Fluorescence recordings presented in Figs. 3 and 4 correspond to averages of four or five interleaved (control and test protocols) trials and were acquired through the 60× Olympus (1.0 NA) objective. Unlike synaptic responses, calcium responses to laser pulses were stable over time, displaying no run down.

Blanchard K., Zorrilla de San Martín J., Marty A., Llano I, & Trigo F.F. (2020). Differentially poised vesicles underlie fast and slow components of release at single synapses. The Journal of General Physiology, 152(5), e201912523.

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