Spleens were collected from immunized animals at the appropriate time points, immediately frozen in OCT, and stored in liquid nitrogen until processing. The cryosections (8 mm thick) were cut along the entire organ to analyze all the planes of the organs. The cryosections were fixed using PBS plus 3% formaldehyde for 10 min at room temperature, washed twice with PBS, and stained using anti-CD35 (IgG purified, BD Pharmigen) or anti-GL-7 (IgM purified, eBioscience) followed by rat anti-IgG Alexa568 (Abcam) or by rat anti-IgM TexasRed (Sigma) respectively. Sections were also stained with anti-CD169 (FITC, Biolegend); the antigen APC-labelled gp120 used for the immunizations was detected in the blue channel. After washing three times with PBS, stained tissue sections were sealed using Gold Antifade reagent (Invitrogen-Life Technologies) and a coverslip. Images were acquired with the Zeiss LSM 510 Confocal Microscope.
Rat anti igm texasred
Manufactured by Merck Group
Rat anti-IgM TexasRed is a laboratory reagent used for the detection and analysis of IgM antibodies in various experimental and analytical applications. It is a monoclonal antibody that specifically binds to the IgM immunoglobulin class and is conjugated with the TexasRed fluorescent dye, enabling visualization and quantification of IgM-positive cells or molecules.
Automatically generated - may contain errors
Lab products found in correlation
Anti gl7, by Thermo Fisher Scientific (2 mentions)
Rat anti igg alexa568, by Abcam (2 mentions)
Gold antifade reagent, by Thermo Fisher Scientific (2 mentions)
Lsm 510 confocal microscope, by Zeiss (2 mentions)
Anti cd169 fitc, by BioLegend (1 mentions)
Anti cd35, by BD (1 mentions)
Background sniper, by Biocare Medical (1 mentions)
2 protocols using rat anti igm texasred
1
Microscopic Analysis of Splenic Germinal Centers
2
Immunofluorescent Analysis of Splenic Germinal Centers
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Spleens were collected from immunized animals at the appropriate time points and cut into ~5-mm-thick pieces, immediately submerged in optimal cutting temperature (O.C.T.) compound (Tissue-Tek, Sakura), snap-frozen using 2-methylbutane (isobutene) and liquid nitrogen, and stored in liquid nitrogen until processing. The cryosections (40 µm thick) were cut along the entire organ to analyze all planes. The cryosections were fixed using ice-cold acetone for 10 min at room temperature and rehydrated in PBS for 5 min. Sections were then blocked by Background Sniper (Biocare) and stained using anti-CD35 (IgG-purified, BD Pharmingen) or anti–GL-7 (IgM-purified, eBioscience), followed by rat anti-IgG Alexa568 (Abcam) or by rat anti-IgM Texas Red (Sigma), respectively. Sections were also stained with anti-CD169 [fluorescein isothiocyanate (FITC), BioLegend]. The antigen APC-labeled gp120 used for the immunizations was detected. After washing three times with PBS, stained tissue sections were sealed using Gold Antifade reagent (Invitrogen–Life Technologies) and a coverslip. Images were acquired with a Zeiss LSM 510 confocal microscope.
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