Egm 2 basal media

The primary human endothelial cells, HUVECs (C2519A) and HPAECs (CC-2530), were from Lonza (Tokyo, Japan). Human pancreatic MIA PaCa-2 and colorectal HCT116 carcinoma cell lines were from the American Type Culture Collection (ATCC, Manassas, VA, USA), and the ovarian cancer RMG-1 cell line was from Japanese Health Science Research Resources Bank (Osaka, Japan). The gemcitabine-resistant human pancreatic Suit2-GR cancer cell line was a gift from Dr. Masao Tanaka (Kyushu University).⁴⁹ (link) Suit2-GR and HCT116 cell lines stably expressing luciferase were established as previously reported.⁵⁰ (link) HUVECs and HPAECs were cultured in EGM-2 basal media (Lonza) supplemented with the SingleQuots Kit (Lonza). HCT116 and RMG-1 cells were cultured in RPMI 1640 (Nacalai Tesque, Tokyo, Japan), and MIA PaCa-2 and Suit2-GR cells were cultured in DMEM (Nacalai Tesque), all supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco, Tokyo, Japan), penicillin (100 U/mL), and streptomycin (100 μg/mL). Cells were cultured at 37°C in a humidified atmosphere equilibrated with 95% air and 5% CO₂.