Proteins used in this work were ferritin and thyroglobulin (Tg) (GE Healthcare, Piscataway, NJ), aldolase, apoferritin and ferritin (Sigma-Aldrich Inc, St. Louis, MO), α-crystallin (Stressgene Biotechnologies, Victoria, British Columbia, Canada) and citrate synthase (Roche Applied Science, Indianapolis, IN).
Citrate synthase
Manufactured by Roche
Sourced in Canada
Citrate synthase is an enzyme that catalyzes the first step in the citric acid cycle, a key metabolic pathway in aerobic organisms. It catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate.
Automatically generated - may contain errors
Lab products found in correlation
Ferritin, by GE Healthcare (2 mentions)
Aldolase, by Merck Group (2 mentions)
Apoferritin and ferritin, by Merck Group (2 mentions)
Thyroglobulin tg, by GE Healthcare (2 mentions)
Malic dehydrogenase, by Merck Group (2 mentions)
Dimethyl malate, by Merck Group (2 mentions)
Malate acid, by Merck Group (2 mentions)
α ketoglutaric acid, by Merck Group (2 mentions)
L glutamic dehydrogenase, by Merck Group (2 mentions)
β actin, by Huabio (2 mentions)
Adenosine diphosphate (adp), by Merck Group (2 mentions)
Dimethyl 2 oxoglutarate, by Merck Group (2 mentions)
Hif 1α, by Abcam (2 mentions)
4 protocols using citrate synthase
1
Diverse Protein Sources in Research
2
Diverse Protein Sources in Research
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Proteins used in this work were ferritin and thyroglobulin (Tg) (GE Healthcare, Piscataway, NJ), aldolase, apoferritin and ferritin (Sigma-Aldrich Inc, St. Louis, MO), α-crystallin (Stressgene Biotechnologies, Victoria, British Columbia, Canada) and citrate synthase (Roche Applied Science, Indianapolis, IN).
3
Metabolic Enzyme Assay Protocol
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Reagents including the following: ADP (Sigma, A5285, St. Louis, USA), NAD (Sigma, N0632), NADH (Sigma, N8129), malate acid (Sigma, 02288), α-ketoglutaric acid (Sigma, 75890), dimethyl malate (Sigma, 374318), and dimethyl 2-oxoglutarate (Sigma, 349631). Enzymes including the following: malic dehydrogenase (Sigma, M2634), L-glutamic dehydrogenase (Sigma, G2501), and citrate synthase (Roche, 2168342). Antibodies including the following: ME2 (Abcam, 139686, Cambridge, USA), HIF-1α (Abcam, 16066), and β-actin (HUABIO, M1210-2, Hangzhou, China).
4
Metabolic Enzyme Activity Assay
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Reagents including the following: ADP (Sigma, A5285); NAD (Sigma, N0632); NADH (Sigma, N8129); malate acid (Sigma, 02288); α-ketoglutaric acid (Sigma, 75890); dimethyl malate (Sigma, 374318); dimethyl 2-oxoglutarate (Sigma, 349631). Enzymes including the following: malic dehydrogenase (Sigma, M2634); L-glutamic dehydrogenase (Sigma, G2501); citrate synthase (Roche, 2168342).
Antibodies including the following: ME2 (Abcam, 139686), HIF-1α (Abcam, 16066), β-actin (HUABIO, M1210-2).
Antibodies including the following: ME2 (Abcam, 139686), HIF-1α (Abcam, 16066), β-actin (HUABIO, M1210-2).
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