8.0 for windows

Frail and robust subjects were compared with respect to the analyzed variables by means of ANOVA one-way tests for continuous Gaussian variables and the Mann–Whitney U test for continuous non-Gaussian variables. Comparisons between baseline and post-stimuli variables were performed by 2-way ANOVA for repeated measures; multiple comparisons were performed by means of Šídák’s multiple comparisons test.
Mitochondrial ATP level and electron chain flux activity were correlated with muscle mass and function by Pearson’s coefficient correlation.
SPSS 27.0 was used for the statistical analyses, and p < 0.05 was considered statistically significant. Graphs were drawn using GraphPad 8.0 for Windows.

As no previous study measured the efficacy of DT in using A.Di.CO and NPI, in the period of the study drawing, the power analysis was conducted using an estimated large effect size (f = 0.40), an alpha level of 0.05, and a power of 0.8. A sample size of 52 is necessary (26 each group) for primary outcomes [42 (link)].
All the analyzed variables were tested for normality by the kurtosis test and they were all normally distributed. Patients randomized to DT were compared to patients randomized to ST by one-way ANOVA for continuous variables and by χ2 test for gender. The effect of DT was evaluated per protocol using the two-way ANOVA for repeated measurements for continuous variables and by χ2 test for trends for the incidence of delirium.
SPSS 25.0 were used for the statistical analyses and p < 0.05 was considered statistically significant. Graphs were drawn using GraphPad 8.0 for Windows.